Clinical analyzer

• US 4,482,251 A
• Filed: 10/19/1981
• Issued: 11/13/1984
• Est. Priority Date: 10/19/1981
• Status: Expired due to Term

• Current Assignee: Schiapparelli Biosystems, Inc.
• Original Assignee: Electro-Nucleonics Incorporated
• Inventors: Saylor, Richard
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US06/312,676
• Time in Patent Office: 1,121 Days
• Field of Search: 356/408-411, 356/414, 356/418, 356/419, 356/432-434, 356/436, 356/440, 422/63, 422/65, 422/67, 422/68, 364/497-499, 364/526
• US Class Current: 356/418
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 2201/0438 Linear motion, sequential
• CPC Class Codes: G01N 2201/0484 Computer controlled

Abstract

A clinical analyzer that is microprocessor controlled is disclosed. A shuttle carries a plurality of cuvettes containing liquids to be spectrophotometrically analyzed by a single beam spectrophotometer. The shuttle moves the cuvettes past the spectrophotometer in a plurality of cycles to complete a given test. At the beginning of a test, spectrophotometer lamp energization is controlled to achieve a predetermined spectrophotometer output for a reference filter. The remaining filters are then positioned one-by-one, and amplifier gain is adjusted to achieve optimum spectrophotometer output for each filter. The gain associated with each filter is stored by the microprocessor, and thereafter during the test, when each filter is used, the stored gain associated with that microprocessor is utilized in the amplifier circuitry. Additionally, at the beginning of each cycle of shuttle movement, an air calibration reading is taken, to be used by the microprocessor in determining absorbance. This air-only reading, in combination with a reference reading taken with a water-filled cuvette at any convenient time, serves to provide for accurate calibration of the system, overcoming problems of drift.

Claims

1. In a clinical analyzer that includes a shuttle carrying a plurality of cuvettes containing liquid to be analyzed spectrophotometrically by a single beam spectrophotometer including a plurality of light filters, means for moving the shuttle past the spectrophotometer so that selected ones of said cuvettes are examined in a plurality of cycles of movement of each cuvette past the spectrophotometer to complete a test run, the improvement for calibrating the spectrophotometer circuitry comprising means for periodically completing a spectrophotometric reading of air only, including an attenuation circuit providing for varying signal attenuation to produce two air-only readings that together determine the slope of a calibration curve, and means for selectively activating said attenuation circuit.

2. A signal analyzer according to claim 1, in which said predetermined attenuation is generally about 3% representing an optical absorbance of about 1.5 absorbance units.

3. A clinical analyzer as in claim 1, in which said activating means activates said attenuation circuit at least once during said plurality of cycles.

4. A clinical analyzer as in claim 3, including a gain control and means active at least at the beginning of a test for completing a reference spectrophotometric reading of each of a plurality of said filters and adjusting said gain control for each filter to achieve a preselected spectrophotometric output, means for storing said gain adjustment for each filter, and means active thereafter during each cycle of testing during said test for adjusting said gain control so that it conforms to the value stored for a given filter when the filter is employed during said test.

5. A clinical analyzer according to claim 4, in which said gain control adjustment means comprises a plurality of analogue switches, various combinations of which are activated to provide for gain adjustment.

6. A clinical analyzer according to claim 5, in which said gain storing and gain adjustment means comprises a microprocessor for storing signals representing said various combinations of said analogue switches providing for said gain adjustment, and interface means coupled to said microprocessor and activating said analogue switches to produce said various combinations during a test.

7. A clinical analyzer as in claim 5, including means for completing a spectrophotometric reading of a cuvette to obtain a reading of a "clear" cuvette for reference purposes.

8. In a clinical analyzer that includes a shuttle carrying a plurality of cuvettes containing liquids to be analyzed past a spectrophotometer, said spectrophotometer having gain control and including a lamp and a filter assembly containing a plurality of light filters, and means for moving the shuttle past the spectrophotometer so that selected ones of said cuvettes are examined in a plurality of cycles of movement of each cuvette past the spectrophotometer so that a plurality of said cycles complete a test for a group of said cuvettes, the improvement for adaptively calibrating the spectrophotometer circuitry comprising means active at least at the beginning of each test for completing a reference spectrophotometric reading of each of a plurality of said filters and adjusting said gain control for each filter to achieve a preselected spectrophotometric output, means for storing said gain adjustment for each filter, and means active thereafter during each cycle of testing during said test for adjusting said gain control so that it conforms to the value stored for a given filter when that filter is employed during said test.

9. A clinical analyzer as in claim 8, in which said adjustment means includes means for first completing a reference spectrophotometric reading of a reference filter and adjusting the energization of said lamp to achieve a predetermined spectrophotometric output, and thereafter completing said spectrophotometric reading of said filters for said gain control adjustment.

10. A clinical analyzer according to claim 8, in which said gain control adjustment means comprises a plurality of analogue switches, various combinations of which are activated to provide for gain adjustment.

11. A clinical analyzer according to claim 10, in which said gain storing and gain adjustment means comprises a microprocessor for storing signals representing said various combinations of said analogue switches providing for said gain adjustment, and interface means coupled to said microprocessor and activating said analogue switches to produce said various combinations during a test.

12. In a system for process monitoring utilizing a source for engergizing a test instrument and detection circuitry coupled to the test instrument and including gain control in a plurality of test modalities, the improvement for adaptively calibrating said system comprising means periodically activated for controlling the energization of said source to an extent so that a predetermined output is achieved by said detection circuitry, means activating each of said modalities when said source is energized to said extent and adjusting said gain control so that the output of said detection circuitry achieves a preselected value, means for storing said gain adjustment for each of said modalities, and means active thereafter during process monitoring for adjusting said gain control to the value stored for a given modality when that modality is employed during process monitoring.