Blood processing system for cell washing
First Claim
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1. Blood processing apparatus for seperation of blood components by centrifugation in a centrifuge rotor comprising:
- a first flexible bag and support means for orienting said first flexible bag with respect to the center of rotation of said rotor to cause, upon rotation of said rotor, lighter density components to accumulate at a first location within said bag and heavier components to accumulate at a second location diagonally opposite said first location and wherein ports are located at said first and second locations;
an input port located at the second location and wherein said support means has a vertically extending wall with a curved surface tilted inwardly toward and eccentric to the axis of the center of rotation.
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Abstract
A cell washing method and apparatus which utilizes centrifugation to separate blood components in a flexible bag. The less dense separated components e.g., supernatant, is expressed from the bag by centrifugal force acting on a plate adjacent the bag and the more dense component e.g., RBC'"'"'s, remains. The bag is oriented at a double angle in the centrifuge so that less dense component accumulates at one location and more dense at a second location diagonally opposite the first location, thus facilitating removal of the less dense and washing of the more dense component.
172 Citations
31 Claims
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1. Blood processing apparatus for seperation of blood components by centrifugation in a centrifuge rotor comprising:
- a first flexible bag and support means for orienting said first flexible bag with respect to the center of rotation of said rotor to cause, upon rotation of said rotor, lighter density components to accumulate at a first location within said bag and heavier components to accumulate at a second location diagonally opposite said first location and wherein ports are located at said first and second locations;
an input port located at the second location and wherein said support means has a vertically extending wall with a curved surface tilted inwardly toward and eccentric to the axis of the center of rotation. - View Dependent Claims (2, 3)
- a first flexible bag and support means for orienting said first flexible bag with respect to the center of rotation of said rotor to cause, upon rotation of said rotor, lighter density components to accumulate at a first location within said bag and heavier components to accumulate at a second location diagonally opposite said first location and wherein ports are located at said first and second locations;
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4. Apparatus for processing fluids in a centrifugal force field to separate constituent components of such fluids comprising in combination:
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(a) a centrifuge having a rotor adapted to rotate at a sufficient speed to cause said components to separate; (b) a first flexible bag mounted on the rotor and adapted to contain a first fluid; (c) a receiver container mounted on the rotor and adapted to receive at least one component of said first fluid; (d) a first conduit means for coupling the flexible bag and the receiver container in fluid communication; (e) a first mass means disposed nearer the center of rotation of the rotor than the flexible bag and adapted to move against a surface of said bag, said mass being sufficient to at least initiate a flow from said bag to said container through said conduit means of component fluid separated in said bag; (f) a first support means for orienting the flexible bag in the rotor such that an output port on said flexible bag is located nearer the axis of the center of rotation of the centrifuge rotor than an input port on said flexible bag whereby during centrifugation, the less dense components will accumulate at the output port and the more dense components at the input port. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. Apparatus for processing fluids in a centrifugal force field to separate constituent components of such fluids comprising in combination:
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(a) a centrifuge having a rotor adapted to rotate at a sufficient speed to cause said components to separate; (b) a plurality of flexible bags in fluid communication with each other adapted to contain a fluid component; (c) each of said bags being disposed in spaces provided between vertically extending walls of a cassette mounted in said rotor; (d) a plurality of mass means, each suspended on one of said walls and adapted to move against a surface of an adjacent bag, said mass being sufficient to at least initiate a flow of component fluid separated in said bag from said adjacent bag to another bag located further away from the center of the rotor; (e) one of said vertically extending walls having a curved surface tilted inwardly toward and eccentric to the axis of the center of rotation of the centrifuge rotor. - View Dependent Claims (16, 17, 18, 19)
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20. A method comprising:
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(a) orienting a flexible bag in a centrifuge against a curved surface support such that when a volume of fluid contained in said flexible bag is rotated in a centrifuge at a speed sufficient to separate said fluid into a less dense and more dense component, the less dense component accumulates at a first port on said bag and the more dense component at a second port on said bag; (b) forcing the less dense component to flow from said flexible bag to a container by applying centrifugal force to a moveable body against a planar surface of said bag while said volume is being rotated; (c) preventing the flow in step (b) until substantial separation occurs in setp (a) and; (d) causing the flow to stop when the less dense component has passed from the first bag to the second bag. - View Dependent Claims (21, 22, 23, 24, 25, 26)
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27. In a process wherein blood is separated into a first blood component and second blood component in a blood processing chamber mounted on a centrifuge rotor and first blood component is thereafter caused to flow through an outlet port of said chamber through a conduit and into a receiver container:
The improvement of causing said less dense and more dense components to accumulate at diagonally opposite inlet and outlet ports on said chamber by orienting the chamber with respect to the axis of the center of rotation of the centrifuge. - View Dependent Claims (28, 29, 30)
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31. Blood processing apparatus for seperation of blood components by centrifugation comprising:
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(a) a first flexible bag wherein lighter density components accumulate at a first location within said bag and heavier components accumulate at a second location diagonally opposite said first location and wherein ports are located at said first and second location; (b) a first fluid conduit fixedly connected to the second location at a lower corner of the first bag, said conduit having a bag spike on one end thereof; and (c) a second bag coupled by a second fluid conduit to said first location at a corner diagonally opposite the lower corner.
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Specification