Biologically active substance, process for preparing the substance and immunoactive composition
First Claim
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1. A biologically active glycoprotein having a molecular weight of 3,000 to 5,000 as determined by a gel filtration method and has activity to inhibit toxoplasma multiplication not only in homologous cells but also in heterologous cells, and further has the following physical and chemical properties;
- (a) solubility;
freely soluble in water and practically incoluble in methanol, ethanol, ether, benzene, chloroform and acetone;
(b) ratio of sugar and protein contents;
protein 85-90%, hexosamine 9-13% and hexose 1-2% calculated as glucose;
(c) constituent amino acid of protein portion;
aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, tryptophan, histidine, arginine and proline;
(d) aspartic acid, glutamic acid and lysine contents in total amino acids;
45 to 65% by weight;
(e) pH;
7.0 to 7.2 (1 W/V % aqueous solution);
(f) thermal stability;
when 1 W/V % aqueous solution of the substance is heated at 60±
0.1°
C. for 30 minutes, the solution still retains the immunoactivity to inhibit toxoplasma multiplication in homologous cells and heterologous cells;
(g) IR absorption;
characteristic absorption at the following wave number (cm-1);
3600-2900 (strong), 1700-1500 (strong), 1440-1380 (medium), 1160-1080 (medium), and 550 (medium);
(h) UV absorption;
maximum absorption is found at 274-276 nm for 0.1 W/V % aqueous solution;
(i) color reaction;
positive Lowry-Folin process, Ninhydrin reaction, Phenol-sulfuric acid reaction and Elson-Morgan'"'"'s reaction;
(j) color and appearance;
white and amorphous.
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Abstract
This invention relates to a biologically active substance which is a glycoprotein having a molecular weight of 3,000 to 5,000 and has activity to inhibit toxoplasma multiplication in homologous cells and heterologous cells.
This invention also relates to a process for preparing the substance, immunoactive compositions containing the substance and use of the substance as a drug.
9 Citations
10 Claims
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1. A biologically active glycoprotein having a molecular weight of 3,000 to 5,000 as determined by a gel filtration method and has activity to inhibit toxoplasma multiplication not only in homologous cells but also in heterologous cells, and further has the following physical and chemical properties;
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(a) solubility;
freely soluble in water and practically incoluble in methanol, ethanol, ether, benzene, chloroform and acetone;(b) ratio of sugar and protein contents;
protein 85-90%, hexosamine 9-13% and hexose 1-2% calculated as glucose;(c) constituent amino acid of protein portion;
aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, tryptophan, histidine, arginine and proline;(d) aspartic acid, glutamic acid and lysine contents in total amino acids;
45 to 65% by weight;(e) pH;
7.0 to 7.2 (1 W/V % aqueous solution);(f) thermal stability;
when 1 W/V % aqueous solution of the substance is heated at 60±
0.1°
C. for 30 minutes, the solution still retains the immunoactivity to inhibit toxoplasma multiplication in homologous cells and heterologous cells;(g) IR absorption;
characteristic absorption at the following wave number (cm-1);
3600-2900 (strong), 1700-1500 (strong), 1440-1380 (medium), 1160-1080 (medium), and 550 (medium);(h) UV absorption;
maximum absorption is found at 274-276 nm for 0.1 W/V % aqueous solution;(i) color reaction;
positive Lowry-Folin process, Ninhydrin reaction, Phenol-sulfuric acid reaction and Elson-Morgan'"'"'s reaction;(j) color and appearance;
white and amorphous.
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2. An immunoactive composition comprising as an active ingredient an immununoactivating effective amount of a biologically active substance in admixture with a pharmaceutically acceptable, non-toxic carrier, the substance being a glycoprotein having a molecular weight of 3,000 to 5,000 as determined by a gel filtration method and having activity to inhibit toxoplasma multiplication not only in homologous cells but also in heterologous cells, and further having the following physical and chemical properties;
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(a) solubility;
freely soluble in water and practically insoluble in methanol, ethanol, ether, benzene, chloroform and acetone;(b) ratio of sugar and protein contents;
protein 85-90%, hexosamine 9-13% and hexose 1-2% calculated as glucose;(c) constituent amino acid of protein portion;
aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, tryptophan, histidine, arginine and proline;(d) aspartic acid, glutamic acid and lysine contents in total amino acids;
45 to 65% by weight;(e) pH;
7.0 to 7.2 (1 W/V % aqueous solution);(f) thermal stability;
when 1 W/V % aqueous solution of the substance is heated at 60±
0.1°
C. for 30 minutes, the solution still retains the immunoactivity to inhibit toxoplasma multiplication in homologous cells and heterologous cells;(g) IR absorption;
characteristic absorption at the following wave number (cm-1);
3600-2900 (strong), 1700-1500 (strong), 1400-1380 (medium), 1160-1080 (medium), and 550 (medium);(h) UV absorption;
maximum absorption is found at 274-276 nm for 0.1 W/V % aqueous solution;(i) color reaction;
positive Lowry-Folin process, Ninhydrin reaction, Phenol-sulfuric acid reaction and Elson-Morgan'"'"'s reaction;(j) color and appearance;
white and amorphous. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10)
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Specification