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Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes

  • US 4,485,175 A
  • Filed: 01/03/1983
  • Issued: 11/27/1984
  • Est. Priority Date: 01/03/1983
  • Status: Expired due to Term
First Claim
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1. A method for the volumetric differentiation of leukocytes into at least two populations which are identified as monocytes and granulocytes, respectively, from a whole blood sample, using an automatic particle analyzing system, said method comprising the steps of:

  • I. supplying, for use by the analyzing system for its mixing and analyzing, the whole blood sample and a volume of an isotonically balanced blood diluent; and

    II. mixing the whole blood sample and the diluent by the analyzing system with a therein supplied lysing reagent in such a manner that the lysing reagent is at a significantly weak concentration and is supplied at a significantly slow rate, so as to subject the leukocytes to a low gradient of lytic shock, so as to result in the volumetric modification of the individual blood cells of at least one of the populations of leukocytes for a significant period of time to thereby enable the automatic differentiation of the leukocyte populations by the analyzing system;

    the lysing reagent comprising a mixture of an aqueous solution of quaternary ammonium salts having surface acting properties.

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