Photometric method for determining courses of reactions

• US 4,492,462 A
• Filed: 02/13/1981
• Issued: 01/08/1985
• Est. Priority Date: 02/16/1980
• Status: Expired due to Fees

• Current Assignee: Franz Muhlbock, Hartmann, Klaus, Jurgen Barry, Wilhelm Pross
• Original Assignee: Franz Muhlbock, Hartmann, Klaus, Jurgen Barry, Wilhelm Pross
• Inventors: Hartmann, Klaus, Barry, Jurgen, Muhlbock, Franz, Pross, Wilhelm
• Primary Examiner(s): ARNOLD, BRUCE
• Application Number:
  US06/234,573
• Time in Patent Office: 1,425 Days
• Field of Search: 356/338-343, 356/434-435, 356/442, 356/436, 356/39, 356/72, 356/73, 350/73, 350/39, 73/64.1
• US Class Current: 356/39
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54

Abstract

The invention provides a photometric method of determining courses of reaction in a specimen, in which method the intensities of the transmitted light and of the stray light are measured, wherein the two intensities are measured simultaneously and the electrical signals corresponding to the transmitted light and to the stray light are combined to form one signal. The invention makes it possible to perform reliable measurement even with low levels of transmitted and stray light.

The method is preferably used in determining the coagulation times of blood. Apparatus for carrying out the method is also disclosed.

Claims

1. A photometric method for determining the coagulation time of a sample, said sample including blood mixed with a reagent, comprising:

detecting the intensity of a light beam transmitted through said sample and forming an electrical signal proportional thereto;

detecting, at an angle of from 90 to 180 degrees to the transmitted light beam, the intensity of light scattered by said sample and forming an electrical signal proportional thereto;

combining said electrical signals to form a combined signal;

from said combined signal, determining the coagulation time of said sample.

2. A method as claimed in claim 1, in which the electrical signals corresponding to the transmitted light beam and to the scattered light are subtracted.

3. A method as claimed in claim 1, in which the electrical signals corresponding to the transmitted light beam and to the scattered light are differentiated and then subtracted to obtain the combined signal.

4. A method as claimed in claim 1, in which the electrical signals corresponding to the transmitted light and to the scattered light are multiplied to obtain the combined signal.

5. A method as claimed in claim 1, in which the scattered light is measured at an angle of 100°

to the transmitted light beam.

6. The method of claim 1, wherein there is included the step of masking the stray light in the vicinity of the region at which the transmitted light beam leaves the region in which scattering occurs.

7. A method as claimed in claim 1, in which the wavelength of the light is chosen in dependence upon the absorption- and/or the scatter behaviour of the particles causing the opacity.

8. A method as claimed in claim 1, in which the maximum of the wavelength of the detected light lies in a range of wavelength of from 350 nm to 550 nm.