Coagulation instrument for performing clotting tests

• US 4,497,774 A
• Filed: 03/08/1983
• Issued: 02/05/1985
• Est. Priority Date: 06/19/1981
• Status: Expired due to Term

• Current Assignee: Medical Laboratory Automation Incorporated
• Original Assignee: Medical Laboratory Automation Incorporated
• Inventors: Scordato, Richard E.
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/473,235
• Time in Patent Office: 700 Days
• Field of Search: 422/64-67, 422/72, 422/73, 422/102, 422/104, 436/69, 356/39, 356/40, 356/246
• US Class Current: 422/73
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54

Abstract

A coagulation instrument for determining clotting times of plasma as an indication of various blood factors. The instrument is adapted to use three or more different reagents which are added selectively to plasma samples in accordance with a code on a cuvette in which the plasma sample is carried through the instrument. Different clotting time tests may be performed sequentially in random order as determined by the coded cuvettes.

Claims

1. A coagulation instrument adapted to randomly perform different clotting time tests on plasma samples in accordance with a test code provided on the cuvette into which a plasma sample is introduced, said instrument comprising:

sensing means for reading a test code on a coded cuvette;

a first dispensing station at which one or more reagents can be dispensed, said first dispensing station having guide means with a plurality of nozzle guides therein;

a test station at which one or more reagents can be dispensed and at which clotting of a reagent plasma mixture is detected, said test station having guide means with a plurality of nozzle guides therein;

means for moving coded cuvettes sequentially from said sensing means to said first dispensing station and thereafter to said test station;

first reservoir and dispensing means adapted to dispense a reagent at said first dispensing station;

first pump means for delivering a predetermined volume of reagent from said first reservoir means at said first dispensing station;

second reservoir and dispensing means adapted to dispense a reagent at said first dispensing station or at said test station;

second pump means for delivering a predetermined volume of reagent from said second reservoir means at said first dispensing station or at said test station;

third reservoir and dispensing means adapted to dispense a reagent at said test station;

third pump means for delivering a predetermined volume of reagent from said third reservoir means at said test station;

said first, second, and third dispensing means each having separate dispensing nozzles with said first dispensing nozzle being adapted to be connected to and cooperate with the first dispensing station guide means, the second dispensing nozzle being adapted to be connected to and cooperate with the first dispensing station guide means or the test station guide means, and the third dispensing nozzle being adapted to be connected to and cooperate with the test station guide means;

circuit means responsive to the dispensing of a reagent into a plasma sample at said test station for detecting clot formation in the reagent plasma mixture and for measuring the time period between reagent dispensing at said test station and clot formation; and

means responsive to the code sensed on a coded cuvette by said sensing means for controlling said first pump means to either dispense or not dispense a reagent to a cuvette from said first reservoir means when the cuvette is moved to said first dispensing station and for controlling said second pump means to either dispense or not dispense a reagent to a cuvette from said second reservoir means when the cuvette is moved to said first dispensing or test station depending on which station guide means the second dispensing nozzle is connected to and for controlling said third pump means to dispense a reagent from said third reservoir means when a cuvette is moved to said test station, the arrangement being such that when reagents are added to a cuvette at said first dispensing station and at said test station in response to the code on the cuvette an APTT test or a double reagent PT test is performed and when a reagent is added to a cuvette only at said test station in response to the code on the cuvette a single reagent PT test is performed.

2. A coagulation instrument according to claim 1 wherein said sensing means includes means for reading a test code on a dual receptacle coded cuvette, and wherein said circuit means includes means for detecting clot formation in each receptacle of said cuvette, and wherein said means responsive to the code on a cuvette for controlling the pump means includes means to dispense a reagent or reagents into both receptacles of the cuvette so that the same test is performed on the reagent plasma mixtures in each receptacle of the cuvette or that different tests are performed on the reagent plasma mixtures in each receptacle.

3. A coagulation instrument according to claim 1 including disposal means by which cuvettes are removed from said cuvette moving means and deposited in a waste container after a reagent plasma mixture therein has been tested.

4. A coagulation instrument according to claim 2 including disposal means by which cuvettes are removed from said cuvette moving means and deposited in a waste container after a reagent plasma mixture therein has been tested.

5. A coagulation instrument according to claim 1 wherein the cuvette moving means comprises stepping means for advancing the cuvettes at a predetermined stepping rate, said stepping rate having a time period greater than the time period of clot formation.

6. A coagulation instrument according to claim 1 including a second dispensing station at which one or more reagents can be dispensed, said second dispensing station having guide means with a plurality of nozzle guides therein, and wherein said first dispensing nozzle is adapted to be connected to and cooperate with said first or said second dispensing station guide means, and wherein said second dispensing nozzle is adapted to be connected to and cooperate with said first or said second dispensing station guide means or said test station guide means.

7. A coagulation instrument according to claim 6 wherein a dispensing nozzle is connected to and cooperates with the first dispensing station when a first predetermined reagent activator time is required for a plasma reagent mixture, and is connected to and cooperates with the second dispensing station when a second predetermined reagent activator time is required for the plasma reagent mixture.

8. A coagulation instrument according to claim 6 wherein the cuvette moving means comprises stepping means for advancing the cuvettes at a predetermined stepping rate, said stepping rate having a time period greater than the time period of clot formation.

9. A coagulation instrument according to claim 6 including disposal means by which cuvettes are removed from said cuvette moving means and deposited in a waste container after a reagent plasma mixture therein has been tested.