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Apparatus for distinguishing multiple subpopulations of cells

  • US 4,499,052 A
  • Filed: 08/30/1982
  • Issued: 02/12/1985
  • Est. Priority Date: 08/30/1982
  • Status: Expired due to Term
First Claim
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1. An apparatus for distinguishing multiple subpopulations of particles, each of which have been labeled with different fluorochromes in pre-determined ratios comprising:

  • means for exciting the fluorochromes on each particle as it flows in a liquid path;

    means for detecting the quantity of fluorescence emitted by the different fluorochromes associated with each particle and determining the ratio of fluorescence quantities of any two fluorochromes associated with each particle; and

    means for classifying said particles into a plurality of subpopulations categories by associating said determined ratios with pre-determined ratios.

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