Polypeptides, a process for their preparation, their use, and a _process for the purification of polypeptides

• US 4,501,733 A
• Filed: 02/16/1983
• Issued: 02/26/1985
• Est. Priority Date: 02/19/1982
• Status: Expired due to Term

• Current Assignee: Ferring Arzneimittel GmbH
• Original Assignee: Ferring Arzneimittel GmbH
• Inventors: Horig, Joachim, Schultheiss, Hartmut
• Primary Examiner(s): Phillips, Delbert R.
• Application Number:
  US06/467,187
• Time in Patent Office: 741 Days
• Field of Search: 260/112.5 R, 424/177
• US Class Current: 514/18.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 5/06043 Leu-amino acid
• CPC Class Codes: C07K 7/22 Tachykinins, e.g. Eledoisins , Substance P; Related peptides

Abstract

Polypeptides of the formula I

V--X--D--Trp--Y--D--Trp--Leu--Z--NH.sub.2

wherein V represents a naturally occurring aminoacid or a physiologically acceptable acid radical, X represents a naturally occurring aminoacid or a single bond, Y represents one of the aminoacids, Phe, Ile, Val, Tyr and L--p--Cl--Phe, and Z represents the aminoacids Met or Nle, and salts thereof, in particular pharmaceutically acceptable salts thereof, a process for the preparation of these polypeptides and pharmaceutical products which contain these compounds. The invention also relates to a process for the purification of polypeptides by column chromatography.

Claims

1. Polypeptides of the formula

View Dependent Claims (2)

2. Polypeptides according to claim 1, wherein V represents Arg or Pro.

3. An analgesic, antiinflammatory antispasmodic or antiptyalistic pharmaceutical composition comprising:

(a) a physiologically compatible drug carrier; and

(b) a therapeutically effective concentration of a composition comprising a polypeptide of the formula

space="preserve" listing-type="equation">V-X-D-Trp-Y-D-Trp-Leu-Z-NH.sub.2

wherein V represents Arg, Lys, Orn or Pro, X represents Gln or a single bond, Y represents one of the aminoacids Phe, Ile, Val, Tyr or L-p-Cl-Phe, and Z represents the aminoacids Met or Nle, or a salt of said polypeptide.

4. A pharmaceutical composition according to claim 3, wherein said amount is sufficient to cause an antispasmodic effect.

5. A pharmaceutical composition according to claim 3, wherein V represents Arg or Pro.