Quantitative analysis film
First Claim
1. An analysis film comprising a reagent layer containing a transaminase substrate composition, a pyruvic acid oxidase composition, a cationic dye-forming color indicator for detecting hydrogen peroxide, and an anionic polymer containing a sulfonate group capable of reacting with formed cationic dye to fix the formed dye.
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Abstract
An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.
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Citations
7 Claims
- 1. An analysis film comprising a reagent layer containing a transaminase substrate composition, a pyruvic acid oxidase composition, a cationic dye-forming color indicator for detecting hydrogen peroxide, and an anionic polymer containing a sulfonate group capable of reacting with formed cationic dye to fix the formed dye.
Specification