Method of using a fibrous lattice

• US 4,505,266 A
• Filed: 04/17/1984
• Issued: 03/19/1985
• Est. Priority Date: 10/26/1981
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Yannas, Ioannis V., Burke, John F.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Samaras, Harrie Stein
• Application Number:
  US06/601,233
• Time in Patent Office: 336 Days
• Field of Search: 128/1 R, 128/155-156, 427/2, 3/1, 604/890-900, 604/266, 424/34, 424/35, 424/94, 424/31, 424/32
• US Class Current: 128/898
• CPC Class Codes: A61F 2310/00365 Proteins; Polypeptides; Degradation products thereof
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: Y10S 623/915 Method or apparatus for preparing biological material

Abstract

This invention relates to the introduction of viable cells into a fibrous lattice by surgical, force-utilizing, or other manipulative techniques, all of which are referred to herein as "seeding." One embodiment comprises an autografting technique which involves intact tissue. Other embodiments which involve the distribution of an aqueous suspension of cells comprise centrifugal, spraying, pipetting and syringe emplacement. Each cell that survives the seeding process may reproduce into a colony of cells which grow until they reach confluence, thereby creating a uniform layer or volume of tissue. By proper placement of cells capable of generating such colonies, the period of recovery of a wound may be drastically reduced. In addition, research by the Applicants indicates that tissue that is regenerated using the methods of this invention to seed a lattice comprising collagen and glycosaminoglycan tends to be smoother, more uniform, less fibrotic, less scarred and more flexible than tissue that is regenerated by wound contraction or conventional autografting.

Claims

1. In the method of using a fibrous lattice which:

a. has controllable biodegradability in the presence of body enzymes;

b. provokes no substantial immune respone or inflammation when grafted or implanted into a wound;

c. has sufficiently high porosity, pore size and permeability to allow cell migration and reproduction; and

d. has sufficiently high strength to resist tearing and collapse if grafted onto a wound, the improvement comprising seeding cells into said lattice.

3. A method of claims 1 or 2 wherein said lattice comprises collagen molecules that are crosslinked and covalently bonded to glycosaminoglycan.

4. A method of claims 1 or 2 wherein, prior to seeding cells into said lattice, said cells are contacted with a substance that increases the rate of reproduction of said cells.

5. A method of claim 3 wherein said glycosaminoglycan is selected from the following group:

chondroitin 6-sulfate, chondroitin 4-sulfate, heparin, heparan sulfate, keratan sulfate, dermatan sulfate, chitin, and chitosan.

2. In the method of using a fibrous lattice which has morphological characteristics that substantially resemble the morphological characteristics of the intercellular proteinous matrix that normally exists within healthy tissue, the improvement comprising seeding cells into said lattice.