ATCC HB8116 And its monoclonal anti-H-Y antibody, Hyclonalan

• US 4,511,661 A
• Filed: 12/30/1983
• Issued: 04/16/1985
• Est. Priority Date: 03/19/1982
• Status: Expired due to Fees

• Current Assignee: University Patents, Inc.
• Original Assignee: University Patents, Inc.
• Inventors: Goldberg, Ellen H.
• Primary Examiner(s): Nucker, Christine M.
• Assistant Examiner(s): Moskowitz, M.
• Application Number:
  US06/567,395
• Time in Patent Office: 473 Days
• Field of Search: 436/501, 436/547, 436/548, 436/503, 436/811, 436/815, 436/824, 436/906, 436/510, 436/518, 436/519, 435/2, 435/68, 435/70, 435/172, 435/240, 435/241, 435/804, 435/948, 435/4, 435/7, 435/29, 424/9, 424/85, 424/177, 260/112 R, 935/104, 935/108, 935/110
• US Class Current: 435/6.16
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: G01N 33/689 related to pregnancy or the gonads
• CPC Class Codes: Y10S 435/948 using viruses or cell lines
• CPC Class Codes: Y10S 435/966 involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 436/821 involving complement factors or complement systems
• CPC Class Codes: Y10S 436/824 Immunological separation techniques
• CPC Class Codes: Y10S 530/806 Antigenic peptides or proteins
• CPC Class Codes: Y10S 530/809 Fused cells, e.g. hybridoma

Abstract

Hybridoma tumor cell line A.T.C.C. No. HB8116. An anti-H-Y antigen monoclonal antibody substance, "Hyclonalan," produced by said cell line. Use of Hyclonalan in immunoselection methodology.

Claims

1. A hybridoma cell line, ATCC HB8166, that produces a monoclonal antibody having specific binding affinity to H-Y antigen.

2. A monoclonal antibody, Hyclonalan, produced by hybridoma cell line ATCC HB8116 and having selective binding affinity to H-Y antigen.

3. An immunological procedure for the identification and separation of male mammalian cells from female mammalian cells comprising:

(a) contacting a mixture of said male and female cells with the monoclonal antibody, Hyclonalan, that has specific binding affinity to H-Y antigen and is produced by hybridoma cell line ATCC HB8116;

(b) allowing a selective immunological reaction to occur between said male cells and Hyclonalan resulting in an antibody-male cell complex; and

(c) detecting said complex and separating said female cells from said complex.

4. The procedure recited in claim 3 wherein said cells identified and separated are selected from the group consisting of mammalian spermatozoan cells and mammalian embryo cells.

5. The procedure recited in claim 3 wherein said detecting and separating step comprises lysing said male cells complexed with Hyclonalan in the presence of a source of complement.

6. The improvement recited in claim 5 wherein said complement source is normal guinea pig serum.

7. An immunological procedure for the identification and separation of male bovine embryos from female bovine embryos comprising:

(a) contacting a mixture of said male and female embryos with the monoclonal antibody Hyclonalan, that has specific binding affinity to H-Y antigen and is produced by hybridoma cell line ATCC HB8116;

(b) allowing a selective immunological reaction to occur between said male embryos and Hyclonalan resulting in an antibody-male embryo complex; and

(c) detecting said complex and separating said female embryos from said complex.

8. The procedure recited in claim 7 wherein said bovine embryos treated are at the 32-cell stage.

9. The procedure recited in claim 7 further comprising reimplanting one or more sex-identified embryos into the uterus of a surrogate for development and birth of a calf of the desired gender.

10. The procedure recited in claim 7 further comprising incubating said embryos with a source of complement after said embryos are treated wity Hyclonalan.

11. The procedure recited in claim 10 wherein said detecting and separating step comprises identifying male embryos by the immunological effect of cytolysis of male cells of said male embryos.

12. An immunological procedure performed on human fibroblast cells for the detection of genetic abnormalities involving expression of the Y chromosome or a fragment thereof comprising:

(a) contacting said cells with the monoclonal antibody, Hyclonalan, produced by hybridoma cell line ATCC HB8116 and having specific binding affinity to H-Y antigen present on the surface of cells expressing the Y chromosome or a fragment thereof;

(b) allowing a selective immunological reaction to occur between said cells and Hyclonalan resulting in an antibody-cell complex; and

(c) detecting and separating said cells expressing the Y chromosome of a fragment thereof from cells lacking said expression.