×

Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays

  • US 4,514,505 A
  • Filed: 05/21/1982
  • Issued: 04/30/1985
  • Est. Priority Date: 05/21/1982
  • Status: Expired due to Term
First Claim
Patent Images

1. An immunoassay for an antigen providing enhanced sensitivity which comprises contacting the antigen under suitable conditions with effective assaying amounts of each of at least two monoclonal antibodies which bind to different antigenic sites on the antigen and which are capable under appropriate conditions in a totally liquid phase of forming a stable complex which includes the antigen and all of the monoclonal antibodies, the monoclonal antibodies being further characterized in that they form a stable complex with the antigen when subjected to gel electrophoresis in the presence of the antigen and when subjected to gel filtration chromatography in the presence of the antigen, and detecting the complex which results from contacting the antigen with the monoclonal antibodies.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×