Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays

• US 4,514,505 A
• Filed: 05/21/1982
• Issued: 04/30/1985
• Est. Priority Date: 05/21/1982
• Status: Expired due to Term

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Moyle, William R., Canfield, Robert E., Ehrlich, Paul H.
• Primary Examiner(s): Padgett, Benjamin R.
• Assistant Examiner(s): Moskowitz, M.
• Application Number:
  US06/380,959
• Time in Patent Office: 1,075 Days
• Field of Search: 436/548, 436/518, 436/540, 436/547, 436/65, 436/86, 436/811, 436/814, 436/815, 436/817, 436/818, 436/500, 436/510, 436/514-516, 436/536, 436/542, 436/804, 424/1, 424/1.5, 435/4, 435/7, 435/68, 435/172, 435/948, 260/112 R, 935/110
• US Class Current: 436/500
• CPC Class Codes: G01N 33/5306 Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G01N 33/577 involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens; G01N33/53 - G01N33/576 take precedence
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
• CPC Class Codes: G01N 33/76 Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
• CPC Class Codes: Y10S 435/962 Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
• CPC Class Codes: Y10S 435/965 involving idiotype or anti-idiotype antibody
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/817 Steroids or hormones
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin

Abstract

Mixtures of monoclonal antibodies which contain effective assaying amounts of each of at least two monoclonal antibodies that bind to different antigenic sites on the antigen and are capable under appropriate conditions of binding simultaneously to an antigen are useful in enhanced sensitivity assays for the antigen. By utilizing such mixtures in diagnostic assays for important antigens such as the polypeptide human chorionic gonadotropin enhanced sensitivity can be achieved as compared with assays employing individual monoclonal antibodies.

Claims

1. An immunoassay for an antigen providing enhanced sensitivity which comprises contacting the antigen under suitable conditions with effective assaying amounts of each of at least two monoclonal antibodies which bind to different antigenic sites on the antigen and which are capable under appropriate conditions in a totally liquid phase of forming a stable complex which includes the antigen and all of the monoclonal antibodies, the monoclonal antibodies being further characterized in that they form a stable complex with the antigen when subjected to gel electrophoresis in the presence of the antigen and when subjected to gel filtration chromatography in the presence of the antigen, and detecting the complex which results from contacting the antigen with the monoclonal antibodies.

2. An immunoassay according to claim 1 which comprises two monoclonal antibodies.

3. An immunoassay according to claim 1, wherein said antigen is a polypeptide and said antigenic sites are amino acid sequences contained therein.

4. An immunoassay according to claim 1, wherein said polypeptide antigen is human chorionic gonadotropin.

5. An immunoassay according to claim 1, wherein said polypeptide antigen is follicle stimulating hormone.

6. An immunoassay according to claim 1, wherein said polypeptide antigen is thyroid stimulating hormone.

7. An immunoassay according to claim 1, wherein said polypeptide antigen is luteinizing hormone.

8. An immunoassay according to claim 1, wherein said effective assaying amounts are amounts of each monoclonal antibody sufficient to permit at least about 10 percent of said antigen present in said sample to form said stable complex.

9. An immunoassay according to claim 1, wherein the amounts of each antibody relative to the amount of other antibodies is in the range from about 10⁶ :

1 to about 1;

10⁶.

10. An immunoassay according to claim 9, wherein the amount is in the range from about 10² :

1 to about 1;

10².

11. An immunoassay according to claim 9, wherein the amount of each antibody relative to the amount of other antibodies is substantially the same as the ratio of the binding constants of the antibodies to the antigen.

12. An immunoassay according to claim 4, which comprises two monoclonal antibodies, one of which binds to the α

chain of human chorionic gonadotropin and the other of which binds to the β

chain of human chorionic gonadotropin.

13. An immunoassay according to claim 12, wherein the antibody which binds to the α

chain is designated A102 or A103 and the antibody which binds to the β

chain is designated B102 to B103.

14. An immunoassay according to claim 4, which comprises two monoclonal antibodies, both of which bind to the β

chain of human chorionic gonadotropin, said antibodies being designated B101 and B102.

15. An immunoassay according to claim 1, wherein at least one of the monoclonal antibodies is adsorbed onto a solid matrix.

16. An immunoassay according to claim 1, wherein the monoclonal antibodies are dissolved in a suitably buffered solution.