Electrochemical hydrogenation of nicotinamide adenine dinucleotide

• US 4,526,661 A
• Filed: 05/31/1983
• Issued: 07/02/1985
• Est. Priority Date: 06/05/1982
• Status: Expired due to Fees

• Current Assignee: BASF AG (BASF SE)
• Original Assignee: BASF AG (BASF SE)
• Inventors: Steckhan, Eberhard, Wienkamp, Rainer
• Primary Examiner(s): Williams, Howard S.
• Assistant Examiner(s): Chapman, Terryence
• Application Number:
  US06/499,261
• Time in Patent Office: 763 Days
• Field of Search: 204/72, 204/73 R, 204/74
• US Class Current: 205/421
• CPC Class Codes: C07H 19/207 the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide
• CPC Class Codes: C25B 3/25 Reduction

Abstract

Nicotinamide adenine dinucleotide (NAD.sup.⊕) is electrochemically hydrogenated to NADH in aqueous solution and in the presence of a metal complex as an electron carrier.

Claims

1. In an electroylsis process wherein nicotinamide adenine dinucleotide (NAD⁺) is hydrogenated to (NADH) by an indirect electrochemical reduction in which the electrolysis is carried out in the presence of an electron carrier, the improvement which comprises:

employing as the electron carrier a metal complex having a reduction potential which is not more negative than -1.3 volt, based on a reference electrode consisting of an Ag/AgCl electrode in saturated aqueous potassium chloride, and wherein the electrolysis is carried out in a cell at a temperature of from about 10°

to 70°

C., a pH of from about 5 to 10 and a current density of from 1 to 100 mA/cm².

2. A process as claimed in claim 1, wherein the metal complex used possesses organic ligands.

3. A process as claimed in claim 1, wherein the metal complex used contains, as the central atom, Rh^I, Rh^III, Ru^I, Ru^III, Ir^I, Ir^III, Fe^II, Fe^O, Ni^II, Ni^O, Co^III or Co^I and, as ligands, 2,2'"'"'-bipyridine, 4,4'"'"'-dimethyl-2,2'"'"'-bipyridine, 1,10-phenanthroline, 2,2'"'"',6'"'"',2"-terpyridine, a tetraazamacrocyclic structure, a porphyrin, a phthalocyanine or NO.