Automated analysis instrument system

• US 4,528,159 A
• Filed: 07/20/1982
• Issued: 07/09/1985
• Est. Priority Date: 07/20/1981
• Status: Expired due to Term

• Current Assignee: Dade Behring, Inc. (Siemens AG)
• Original Assignee: American Hospital Supply Corp. (Baxter International Inc.)
• Inventors: Liston, Max D.
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/400,191
• Time in Patent Office: 1,085 Days
• Field of Search: 422/63-67, 422/100, 422/99, 422/102, 435/805, 356/246, 356/244
• US Class Current: 422/65
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 2035/023 forming cuvettes in situ, e.g. from plastic strip
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

An automated instrument system for analyzing constituents of a patient sample while reacting a reagent specific for the selected constituent within the sample. The analyzer includes: a continuous flexible cuvette belt having a series of parallel discrete reaction compartments thereon, a carousel for dispensing solid tabletted reagents into the compartments, a means for effecting ultrasonic dissolution of the tabletted reagent in the reaction compartment, means for dispensing sample from a source thereof into a reaction compartment, including a sample source transport carousel assembly, and means for transporting the cuvette belt through a plurality of analysis stations located in a linear relationship along the path of travel of the cuvette belt.

Claims

1. An automated instrument system for analyzing the constituents of a patient sample while reacting a reagent specific for the selected constituent within said sample, the system comprising:

(a) a continuous flexible cuvette belt comprising a series of parallel, discrete reaction compartments formed in spaced relationship therein, each of said discrete reaction compartments being substantially optically transparent, having an open top end and a closed bottom end and adapted for engagement by a cuvette track;

(b) carousel means for dispensing solid tabletted reagents into said discrete reaction compartments of such cuvette belt, said tabletted reagents being stored in a series of tablet dispensers within the carousel;

(c) means for effecting ultrasonic dissolution of said tabletted reagents, said means being positioned along a cuvette track between the carousel means for dispensing solid tabletted reagents and the means for dispensing sample;

(d) means for dispensing sample from a patient sample container into said discrete reaction compartments of said cuvette;

(e) means for transport of the patient sample container including a carousel assembly located downstream from said reagent dispenser, said carousel assembly comprising;

(i) a loading carousel into which patient sample containers can be randomly loaded,

(ii) a transfer carousel which accepts patient sample containers from the loading carousel and positions them in cooperative relation with the sample dispensing means, said transfer carousel including means for identifying said patient sample container from coded information contained on said sample container, and

(iii) an unloading carousel which receives patient sample containers from the transfer carousel subsequent to transfer of a portion of the sample to a discrete reaction compartment of the cuvette belt;

(f) a plurality of analysis stations arranged in linear relationship to one another along a track for advancement of the cuvette belt; and

(g) means for transporting, at a pre-determined rate, said cuvette belt along a continuous cuvette tract past a plurality of analysis stations.

2. The analysis system of claim 1 wherein said cuvette transport means comprises a track having a means disposed therein for engaging and advancing said cuvette compartments.

3. The analysis system of claim 2 wherein at least a portion of said cuvette transport track is disposed in means defining a water bath for maintaining said reagent and sample reaction mixture at a predetermine incubation temperature.

4. The analysis system of claim 2 wherein said cuvette compartment engaging and advancing means advances said reaction compartments in a stepped manner whereby said reaction compartments are held stationary at said analysis stations for a fixed period of time before being advanced to their next stepped position.

5. The analysis system of claim 4 wherein the number of stepped positions of said reaction compartments between said analysis stations corresponds to desired periods during the reagent reaction times for analyzing said selected constituents.

6. The analysis system of claim 1 further comprising a means for dividing said cuvette belt into sections having a number of reaction compartments corresponding to the number of analysis reactions to be performed at a given time.

7. The analysis system of claim 6 wherein said dividing means comprises cuvette belt cutter.

8. The analysis system of claim 6 further comprising a means for feeding said cuvette belt sections into said cuvette transport track.

9. The analysis system of claim 8 wherein said cuvette feeding means comprises a portion of said cuvette transport track having a separate cuvette belt engaging and advancing means disposed therein.

10. The analysis system of claims 6 wherein said cuvette belt dividing means is operated in response to said sample identifying means.

11. The analysis system of claim 10 further comprising a means for feeding said cuvette belt sections into said transporting means in response to said sample identifying means.

12. The analysis of claim 1 wherein said analysis stations comprises a photo-optical system to analyze said reagent and sample reaction.

13. The analysis system of claim 12 wherein said photo-optical system is bichromatic.

14. The analysis system of claim 12 wherein said photo-optical system comprises a single light source for generating selected wavelengths of light and separate light guides for transmitting said light wavelengths to each of said analysis stations.

15. The analysis system of claim 14 wherein said light guides are fiber optical bundles.

16. The analysis system of claim 14 wherein said light guides are fluid filled light pipes.

17. The analysis system of claim 14 further comprising common wavelength selective filters for sequentially transmitting said selected wavelengths of light through said light guides to each of said analysis stations.

18. The analysis system of claim 17 wherein said common wavelength selective filters are segments of a rotary source filter wheel, the selected wavelength of light output of each filter segment being sequentially directed to said separate light guides for transmission to said analysis stations.

19. The analysis system of claims 17 wherein said different wavelengths of light transmitted to each of said analysis stations are selected to response to said sample identifying means.

20. The analysis system of claim 14 further comprising a second set of separate light guides for directing the outputs of each said analysis stations to photodetector means.

21. The analysis system of claim 20 further comprising a second set of common wavelength selective filters sequentially intercepting the outputs of said separate light guides before being directed to said photodetector means.

22. The analysis system of claim 21 wherein said second set of common wavelength selective filters are segments of a rotary detector filter wheel.

23. The analysis system of claims 22 wherein said source and detector filter wheels are rotated in aligned synchronism with each other.

24. The analysis system of claim 23 wherein said source and detector filter wheels have identical filter segments.

25. The analysis system of claim 1 wherein said reagent is in a solid form.

26. The analysis system of claim 25 wherein said solid reagent is formed into a single tablet.

27. The analysis system of claim 26 wherein said reagent tablet is stored in a dispenser containing a number of identical reagent tablets, said dispenser being adapted to drop said tablets one at a time into said cuvette reaction compartments.

28. The analysis system of claim 27 further comprising means positioned along the cuvette track subsequent to the tablet dispensing means and in advance of the ultrasonic dissolution means for dispensing diluent into said cuvette compartment containing tabletted reagent.

29. The analysis system of claim 28 further comprising a means for mixing said reagent tablet and diluent in said compartment.

30. The analysis system of claim 29 wherein said mixing means comprises an ultrasonic horn.

31. The analysis system of claims 30 wherein said ultrasonic horn is disposed within said water bath.

32. The analysis system of claims 28 wherein said diluent is dispensed into said cuvette compartment in response to said sample identifying means.

33. The analysis system of claim 1 further comprising a means for identifying each of said samples.

34. The analysis system of claim 33 wherein multiple different reagents are contained in said instrument and said reagent dispensing means further comprises a means for selecting one or more of said reagents for dispensing into said cuvette compartment in response to said sample identifying means.

35. The analysis system of claims 33 wherein said sample is dispensed into said cuvette compartment in response to said sample indentifying means.

36. The analysis system of claims 33 wherein the output of said photodetector means is read in response to said sample identifying means.

37. The analysis system of claim 36 further comprising a means to store the output readings of said photodetector means.

38. The analysis system of claim 37 further comprising a means to calculate the concentration of said selected sample consituent from the photometer output readings in said storage means.

39. The analysis system of claim 38 wherein said calculating means selects certain of said photometer output readings in said storage means corresponding to three or more adjacent analysis stations to establish absorption deltas for zero order kinetic reactions.

40. The analysis system of claim 39 wherein said calculating and storage means comprise a microprocessor.