Preparation of an entomopathogenic fungal insect control agent

• US 4,530,834 A
• Filed: 09/17/1982
• Issued: 07/23/1985
• Est. Priority Date: 09/17/1982
• Status: Expired due to Fees

• Current Assignee: United States Department Of Agriculture
• Original Assignee: United States Department Of Agriculture
• Inventors: McCabe, Dennis, Soper, Richard S.
• Primary Examiner(s): Wiseman, Thomas G.
• Assistant Examiner(s): Teskin, Robin Lyn
• Application Number:
  US06/419,637
• Time in Patent Office: 1,040 Days
• Field of Search: 435/242, 435/243, 435/254, 435/911, 424/93, 424/195
• US Class Current: 424/93.5
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom
• CPC Class Codes: C12N 1/04 Preserving or maintaining viable microorganisms immobilised microorganisms C12N11/00
• CPC Class Codes: Y10S 435/911 using fungi

Abstract

Entomopathogenic fungi useful in the control of insects is prepared by culturing mycelia in a suitable medium, harvesting the growing mycelia, treating the mycelia with a protective agent, drying the treated mycelia and grinding and storing the dried product.

Claims

1. A process for preparing dried mycelia for use as an insect control agent comprising culturing mycelia in a suitable medium, harvesting growing mycelia, treating said mycelia with a chemical protectant to prevent excessive loss of viability during storage, incubating the treated mycelia for about four to five hours at about 22°

to 26°

C. and about 20 to 40% relative humidity, drying the incubated mycelia at about 4°

C. with rapid air circulation for about 18 hours, drying further the partially dried mycelia at ambient room temperature of about 20°

to 26°

C. with rapid air flow for about four to six hours and then grinding and storing the dried mycelia.

6. The ground dried mycelial product produced by the process of claim 1.

2. A process for preparing dried mycelia for use as an insect control agent comprising culturing mycelia in a suitable fermentation medium, harvesting the mycelia while it is in active growth phase by filtering the fermentation medium on a suitable mesh screen to obtain thin mycelial mats, treating the mats with a 10% w/v aqueous solution of maltose, glucose or similar agent to saturation, incubating the treated mats for about four to five hours at about 22°

to 26°

C. and about 20 to 40% relative humidity, drying the incubated mycelial mats at about 4°

C. and a relative humidity of about 95 to 100% with rapid air circulation for about 18 hours, drying further the partially dried mats at ambient room temperature of about 20°

to 26°

C. with rapid air flow for about four to six hours at a drying rate of about 60 g/ft² /hr and grinding the mycelia to a powder.

3. The process of claim 2 wherein the cultured mycelia is collected on an 18-mesh screen in mats about 1 to 2 mm thick.

4. The process of claim 3 wherein the cultured mycelia is derived from a fungus of the order Entomophthorales.

5. A method of controlling insect populations on plants comprising growing a culture of a fungus of the order Entomophtorales on a suitable fermentation medium, filtering the medium while the fungi are actively growing to obtain a thin mycelial mat, treating the mycelial mat by spraying it to saturation with a chemical protectant to prevent excessive loss of viability during storage, incubating the treated mat for about four to five hours at about 22°

to 26°

C. and about 20 to 40% relative humidity, drying the incubated mycelial mat at about 4°

C. with rapid air circulation for about 18 hours, drying further the partially dried mycelia at ambient room temperature of about 20°

to 26°

C. with rapid air flow for about four to six hours, grinding the dried mycelial mat to a powder, formulating the dried mycelia as a wettable powder and spraying the wettable powder on plants.

7. Mycelia in stable, dry, powdered form, said mycelia having been prepared by culturing mycelia in a suitable medium, harvesting growing mycelia, treating said mycelia with a chemical protectant to prevent excessive loss of viability during storage, incubating the treated mycelia for about four to five hours at about 22°

to 26°

C. and about 20 to 40% relative humidity, drying the incubated mycelia at about 4°

C. with rapid air circulation for about 18 hours, drying further the partially dried mycelia at ambient room temperature of about 20°

to 26°

C. with rapid air flow for about four to six hours and grinding the dried mycelia, said mycelia being wettable and useful as an insect control agent.

8. The mycelia of claim 7 prepared from the mycelia of a fungus of the order Entomophthorales.