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Acidic polysaccharide CH-1 isolated from Chlorella pyrenoidosa and the use thereof

  • US 4,533,548 A
  • Filed: 11/15/1982
  • Issued: 08/06/1985
  • Est. Priority Date: 12/02/1981
  • Status: Expired due to Fees
First Claim
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1. An acidic polysaccharide having the following properties:

  • (a) consisting of carbon, hydrogen, oxygen and nitrogen with an approximate elementary analysis of O;

    54.05%;

    C;

    38.49%;

    H;

    6.07%; and

    N;

    1.39%;

    (b) a sedimentation coefficient of S20,w=6.15 S when determined by ultracentrifugation;

    (c) impermeability to cellophane membrane,(d) a melting point characterized by coloration at about 240°

    C. with carbonization at higher temperatures;

    (e) a specific rotation of [α

    ]D29 =-9.52 (c=1, H2 O);

    (f) solubility in water, and insolubility in methanol;

    (g) a positive color reaction with anthrone, Molisch, and carbazole-H2 SO4 and a negative color reaction with Folin-Lowry and ninhydrin;

    (h) no detectable protein as measured by Folin-Lowry color reaction test;

    (i) a monosaccharide analysis consisting of rhamnose, galactose, arabinose, glucose and glucuronic acid when detected by paper chromatography and gas chromatography after hydrolysis of said polysaccharide in 7% H2 SO4 under refluxing for two hours;

    (j) a UV spectrum with end absorption in 0.125% aqueous solution;

    (k) an IR spectrum (KBr tablet) with absorption bands at 3420, 2930, 1640, 1380, 1125, 1065, 1040, 990, 915, 835 and 880 cm-1 ;

    (l) an electrophoretic pattern on acetate membrane as shown in FIG. 2, electrophoresced to 0.8 cm for (+) with 0.1M pyridine-acetic acid at pH 5.0, 200 V (4 mA) for 20 minutes;

    (m) an ultra-centrifuge pattern as shown in FIG. 3;

    (n) being obtained as a white powdery precipitate; and

    (o) having a sugar content of 53% when calculated as glucose.

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