Method for measurement of liquid samples
First Claim
1. In a method for assaying an analyte comprising applying a liquid sample having a relatively high viscosity containing the analyte onto a spreading layer of a multilayer analysis element comprising a support having laminated thereon, in sequence, at least one reagent layer containing a substance capable of converting a detectable chemical species upon reaction with said analyte and a liquid sample-spreading layer and then detecting said detectable chemical species, the improvement which comprises applying a wetting liquid onto said spreading layer of the multilayer analysis element prior to applying said liquid sample to the multilayer analysis element, said liquid sample being applied to the multilayer analysis element while at least the surface of said spreading layer is maintained wet with said wetting liquid.
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Abstract
In a method for assaying an analyte comprising applying a liquid sample containing an analyte to a spreading layer of a multilayer analysis element comprising a support having laminated thereon at least one reagent layer containing a substrate capable of converting a detectable chemical species upon reaction with the analyte and a liquid sample-spreading layer and then detecting the chemical species, a wetting liquid is applied onto the spreading layer, prior to applying the liquid sample to the spreading layer. A liquid sample having a high analyte content can be measured with high accuracy.
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Citations
23 Claims
- 1. In a method for assaying an analyte comprising applying a liquid sample having a relatively high viscosity containing the analyte onto a spreading layer of a multilayer analysis element comprising a support having laminated thereon, in sequence, at least one reagent layer containing a substance capable of converting a detectable chemical species upon reaction with said analyte and a liquid sample-spreading layer and then detecting said detectable chemical species, the improvement which comprises applying a wetting liquid onto said spreading layer of the multilayer analysis element prior to applying said liquid sample to the multilayer analysis element, said liquid sample being applied to the multilayer analysis element while at least the surface of said spreading layer is maintained wet with said wetting liquid.
Specification