Coupling of nucleic acids to solid support by photochemical methods
First Claim
1. A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) a phenanthridinium halide, and (3) photochemically reactive derivatives of (1) and (2), and (c) a divalent radical chemically linking the solid substrate (a) and the member (b).
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Accused Products
Abstract
A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of a furocoumarin, a phenanthridium halide, and photochemically reactive derivatives thereof, and (c) a divalent radical chemically linking the substrate and the member (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol diglycidyl ether to an amino-substituted angelicin or psoralen or phenanthridinium bromide which in turn is photochemically linked to a nucleic acid. This is capable of hybridizing with other nucleic acid fragments and is thereby useful in diagnostic assays.
158 Citations
16 Claims
- 1. A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) a phenanthridinium halide, and (3) photochemically reactive derivatives of (1) and (2), and (c) a divalent radical chemically linking the solid substrate (a) and the member (b).
- 9. A hybridization probe comprising (a) a solid substrate, (b) a member selected from the group consisting of (1) a furocoumarin, (2) ethidium bromide, and (3) photochemically reactive derivatives of (1) and (2), (c) a divalent radical chemically linking the solid substrate (a) and the member (b), and (d) a nucleic acid photochemically linked to member (b) and fixed thereby to the solid substrate (a).
Specification