Instrument for measuring the hematocrit value of blood

• US 4,547,735 A
• Filed: 01/24/1983
• Issued: 10/15/1985
• Est. Priority Date: 01/23/1982
• Status: Expired due to Fees

• Current Assignee: Holger Kiesewetter
• Original Assignee: Holger Kiesewetter
• Inventors: Kiesewetter, Holger, Myrenne, Heinz, Mussler, Klaus, Lazar, Hartmut
• Primary Examiner(s): Karlsen, Ernest F.
• Assistant Examiner(s): O'Shea, Kevin D.
• Application Number:
  US06/460,565
• Time in Patent Office: 995 Days
• Field of Search: 324/71.1, 324/71.4, 324/439, 324/442, 324/425, 324/447, 324/450, 324/62 R, 204/403, 204/400
• US Class Current: 324/450
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

The new instrument for taking readings of the hematocrit value from a blood sample has two electrodes with which the conductivity of the sample may be measured. The sample contacting faces of the electrodes are placed in a horizontal plane with an accurately fixed spacing therebetween so that a blood column of a given size may be formed from the sample. The sample is acted upon by ac. From a reading representative of the change in impedance the hematocrit value may be read off from a calibration curve.

Claims

1. An apparatus for measuring the hematocrit value of a blood sample comprising:

an upper electrode having a sample contact surface;

a lower electrode having a sample contact surface which is substantially the same size and shape as said upper electrode contact surface;

means for selectively moving the electrodes between a blood sample measurement position and a second position where said electrodes are displaced away from each other to facilitate cleaning of the electrode faces and introduction of blood samples onto the electrode faces and wherein said electrode faces in said sample measurement position are substantially parallel with said upper electrode face being located directly above said lower electrode face at a spaced distance from said lower electrode face such that a blood sample defining a cylindrical column having a vertical axis may be formed between said upper and lower electrode faces, said electrode faces being oriented such that sedimentation of said blood sample occurs along the vertical axis of said blood sample column; and

conductometry means connected to said electrodes for measuring the hematocrit value of the blood sample.

2. The apparatus as recited in claim 1 wherein the means for moving the electrodes comprises:

a fixed base having a substantially horizontal, planar upper surface mounting said lower electrode such that the contact surface of the lower electrode faces upward and is substantially parallel with the base'"'"'s upper surface and upon which the sample to be measured may be placed;

a movable cover having a substantially planar, lower surface mounting said upper electrode such that the contact surface of the upper electrode faces downward and is substantially parallel with the cover'"'"'s lower surface; and

,

hinge means which rotatably connects the cover to the base for raising and lowering the cover with respect to the base to move the electrodes between said sample measurement position and said second position.

3. An apparatus is recited in claim 1 one wherein the conductometry means comprises:

an oscillator means for applying an alternating voltage to the upper and lower electrodes;

means for measuring the conductivity of the blood sample column located between the upper and lower electrodes and converting its value into a DC signal that is proportional to the conductivity;

means for comparing the conductivity value to those of known samples and converting the value into a reading for the hematocrit; and

means for displaying the hematocrit value measured.

4. The apparatus as recited in claim 1 wherein said electrode contact surfaces are substantially circular in aspect and have an area of from 10 to 100 SQ. MM.

5. The apparatus as claimed in claim 4 wherein said area is generally between 20 and 50 sq. mm.

6. The apparatus as recited in claim 1 wherein the electrodes consist of a material that is electrically conductive, non-corrosive and non-reactive with blood.

7. The apparatus as recited in claim 6 wherein the electrodes are made from a metal selected from the following group:

platinum, silver, gold, titanium, nickel, stainless steel and chromium.

8. The apparatus as recited in claim 1 wherein the electrodes are made from an electrically-conductive material and have sample contact surfaces that are plated with a material that is electrically-conductive, noncorrosive and non-reactive with blood.

9. The apparatus as recited in claim 1 wherein the electrode contact surfaces are plated with a metal selected from the following group:

platinum, silver, gold, titanium, nickel, stainless steel and chromium.

10. The apparatus as recited in claim 1 wherein the fixed distance between the sample contact surfaces in said sample measurement position is from 0.5 to 3 mm.

11. The apparatus as recited in claim 10 wherein the fixed distance is from 1.4 to 2.2 mm.

12. The apparatus as recited in claim 2 further comprising switch means for activating the conductometry means when the cover is moved to said sample measurement position.

13. The apparatus as recited in claim 1 further comprising:

means for controlling the temperature of the blood sample.

14. The apparatus as recited in claim 1 wherein the oscillator means supplies 0.1 to 0.7 Vac rms.

15. The apparatus as recited in claim 14 wherein the oscillator means operates at a frequency of from 1.8 to 6 kHz.