Element for electrophoresis

• US 4,548,869 A
• Filed: 03/12/1984
• Issued: 10/22/1985
• Est. Priority Date: 03/11/1983
• Status: Expired due to Term

• Current Assignee: Director of The Finance Division Minister's Secretariat Science and Technology Agency
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Ogawa, Masashi, Shiraishi, Hisashi, Ikeda, Teppei
• Primary Examiner(s): Ives, Patrick C.
• Application Number:
  US06/588,752
• Time in Patent Office: 589 Days
• Field of Search: 428/483, 428/474.7, 428/475.8, 428/476.6, 428/532, 526/287, 526/303.1, 524/521, 204/299 R, 204/180 G
• US Class Current: 204/616
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10T 428/31507 Of polycarbonate
• CPC Class Codes: Y10T 428/31623 Next to polyamide or polyimide
• CPC Class Codes: Y10T 428/31728 Next to second layer of polyamide
• CPC Class Codes: Y10T 428/31743 Next to addition polymer from unsaturated monomer[s]
• CPC Class Codes: Y10T 428/31746 Polymer of monoethylenically unsaturated hydrocarbon
• CPC Class Codes: Y10T 428/31754 Natural source-type polyamide
• CPC Class Codes: Y10T 428/31783 Paper or wood
• CPC Class Codes: Y10T 428/31797 Next to addition polymer from unsaturated monomers
• CPC Class Codes: Y10T 428/31971 Of carbohydrate

Abstract

An element for electrophoresis comprising the following three-layer structure laminated in the order:

(I) a support layer;

(II) an adhesive layer comprising a polymer having at least one specifically selected repeating unit; and

(III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, and a compound containing at least one carbamoyl group (modifier).

Claims

1. An element for electrophoresis comprising the following three-layer structure laminated in the order:

(I) a support layer;

(II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of;

(1) a repeating unit having the formula (1);

##STR18## in which R¹¹ is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

Q¹ is --COO--, --CON(R¹¹)--, or an arylene group containing 6-10 carbon atoms;

L¹ is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R¹¹)-- and containing 3-15 carbon atoms, or a divalent atom containing at least one linkage selected from the group consisting of --O--, --N(R¹¹)--, --CO--, --SO--, --SO₂ --, --SO₃ --, --SO₂ N(R¹¹)--, --N(R¹¹)CON(R¹¹) and --N(R¹¹)COO--, and containing 1-12 carbon atoms, in which R¹¹ has the same meaning as defined above;

R¹² is --CH═

CH₂ or --CH₂ CH₂ X¹, in which X¹ is a substituent replaceable with a nucleophilic group or releasable in the form of HX¹ by a base; and

x¹ and y¹ both representing molar percents range from 0 to 99 and from 1 to 100, respectively, and x¹ +y¹ is not less than 90;

(2) a repeating unit having the formula (2);

##STR19## in which R²¹ is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

R²² is --CH═

CH₂ or --CH₂ CH₂ X², in which X² is a substituent replaceable with a nucleophilic group or releasable in the form of HX² by a base;

L² is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ² --, and --COZ² R²³ --, in which R²³ is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z² is the oxygen atom or NH; and

x² and y² both representing molar percents range from 10 to 95 and from 5 to 90, respectively, and x² +y² is not less than 90; and

(3) a repeating unit having the formula (3);

##STR20## in which R³¹ is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

L³ is a divalent linkage group containing 1-20 carbon atoms;

X³ is an active ester;

x³ and y³ both representing molar percent range from 0 to 95 and from 5 to 100, respectively, and x³ +y³ is not less than 90; and

m is 0 or 1; and

(III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a cross-linking agent in the presence of water, and a compound containing at least one carbamoyl group serving as modifier.

2. The element for electrophoresis as claimed in claim 1, in which said medium layer for electrophoresis contains a water-soluble polymer and agarose.

3. The element for electrophoresis as claimed in claim 1 or 2, in which said compound serving as modifier is urea or formamide.

4. The element for electrophoresis as claimed in claim 1 or 2, in which said support layer is made of a plastic material sheet.

5. The element for electrophoresis as claimed in claim 4, in which the plastic material sheet is a polyethylene terephthalate sheet.

6. The element for electrophoresis as claimed in claim 1, in which the adhesive layer consists essentially of a polymer having the formula (1).