Optical sensor with beads

• US 4,557,900 A
• Filed: 09/28/1982
• Issued: 12/10/1985
• Est. Priority Date: 09/28/1982
• Status: Expired due to Term

• Current Assignee: Terumo Cardiovascular Systems Corporation (Terumo Corporation)
• Original Assignee: Cardiovascular Decisions, LLC
• Inventors: Heitzmann, Harold A.
• Primary Examiner(s): Turk, Arnold
• Assistant Examiner(s): Hill, Jr., Robert J.
• Application Number:
  US06/425,420
• Time in Patent Office: 1,169 Days
• Field of Search: 422/55, 422/56, 422/58, 422/86, 422/87, 422/91, 436/68, 436/165, 436/167, 436/168, 436/169, 436/170, 436/178, 436/127, 436/133, 436/136, 436/172
• US Class Current: 422/400
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3

Abstract

An optical sensor comprising a selectively permeable matrix of hydrophobic material and a plurality of hydrophilic beads dispersed in the matrix. At least some of the beads carry an optical indicator. The matrix is capable of transmitting light at least at selected wavelengths from outside the matrix to the beads.

Claims

1. An optical sensor comprising:

a matrix of hydrophobic material, said matrix being permeable to at least a selected gas in a liquid and substantially impermeable to the liquid;

a plurality of particles of hydrophilic material dispersed in said matrix, at least some of said particles carrying an optical indicator in solution which is capable of responding to said selected gas; and

said matrix being capable of transmitting light at least at selected wavelengths from outside said matrix to said particle, and said matrix isolating the particles from the liquid so that the optical indicator can respond to said light and provide an optical signal related to partial pressure of said selected gas.

2. An optical sensor as defined in claim 1 wherein each of said particles which carries said indicator has an outer surface and interior voids opening at said outer surface, and at least some of said indicator is carried in said voids of said particles, said particles which carry said indicator are capable of transmitting light at said selected wavelengths.

3. An optical sensor as defined in claim 1 wherein substantially all of said particles are completely enclosed within said matrix and are not exposed at the surface of said matrix.

4. An optical sensor as defined in claim 1 wherein said hydrophilic material is polyacrylamide.

5. An optical sensor as defined in claim 1 wherein said matrix is arranged in a thin membrane-like layer.

6. An optical sensor as defined in claim 1 wherein said hydrophobic material is silicone.

7. An optical sensor as defined in claim 1 wherein said particles which carry the indicator are substantially spherical.

8. An optical sensor as defined in claim 1 wherein the largest cross-sectional dimension of any of said particles is less than the thickness of said matrix.

9. An optical sensor as defined in claim 1 wherein said particles are substantially evenly dispersed throughout said matrix.

10. An optical sensor as defined in claim 1 wherein said selected gas is carbon dioxide and said matrix is also permeable to oxygen and the optical indicator carried by a first group of said particles is capable of responding to carbon dioxide and the optical indicator carried by a second group of said particles is capable of responding to oxygen whereby the optical sensor responds to both oxygen and carbon dioxide.

11. An optical sensor as defined in claim 1 wherein said matrix is constructed of silicone, said particles are constructed of polyacrylamide, said optical indicator is a fluorescent optical indicator, and said matrix and said particles are arranged in a thin membrane-like layer.

12. An optical sensor as defined in claim 1 wherein said solution is an aqueous solution.

13. An optical sensor as defined in claim 12 wherein said aqueous solution includes a catalyst to hasten the response of the optical indicator to said selected gas.

14. An optical sensor comprising:

a selectively permeable matrix of hydrophobic material, said matrix being permeable to at least a selected gas and substantially impermeable to water;

a plurality of particles of hydrophilic material dispersed in said matrix, at least some of hydrophilic particles carrying an optical fluorescent indicator in solution, said indicator being capable of responding to the selected gas that permeates the matrix substantially all of said particles being completely enclosed by said matrix; and

said matrix being capable of transmitting light at least at selected wavelengths whereby the fluorescent indicator can provide a fluorescent optical signal related to a characteristic of the selected gas and the optical signal can be transmitted out of the matrix.

15. An optical sensor as defined in claim 14 wherein said hydrophilic material is selected from the group consisting of polyacrylamide, cross-linked dextran, agarose, poly(hydroxyalkyl methacrylate), and porous glass.

16. An optical sensor as defined in claim 14 wherein each of said particles which carries said indicator has an outer surface and interior voids opening at said outer surface, and at least some of said indicator is carried in said voids of said particles.

17. An optical sensor as defined in claim 16 wherein said hydrophobic material is silicone.

18. An optical sensor as defined in claim 17 wherein said solution is an aqueous solution.

19. An optical sensor as defined in claim 18 wherein said particles are substantially evenly dispersed throughout said matrix.

20. An apparatus for measuring the partial pressure of at least one gas within a liquid, said apparatus comprising:

a membrane of hydrophobic material, said membrane being permeable to at least one gas and substantially impermeable to a liquid, said membrane being adapted to be positioned in contact with said liquid;

a plurality of hydrophilic particles dispersed in said membrane, at least some of said particles carrying an optical indicator in solution which is capable of responding to said at least one gas, said matrix isolating the particles from the liquid;

means for directing light toward said membrane;

said membrane being capable of transmitting at least some of such light to said particles whereby the optical indicator can respond to said light and provide an optical signal related to partial pressure of said at least one gas; and

means for transmitting said optical signal away from said membrane.

21. An apparatus as defined in claim 20 including a wall with a recess therein, said membrane being in said recess, and said wall being capable of transmitting at least some of the light from the directing means to the membrane.