Apparatus for preparing and embedding tissue samples for histological examination

• US 4,569,647 A
• Filed: 09/16/1983
• Issued: 02/11/1986
• Est. Priority Date: 09/16/1983
• Status: Expired due to Term

• Current Assignee: Pelam, Inc.
• Original Assignee: Pelam, Inc.
• Inventors: McCormick, James B.
• Primary Examiner(s): Woo, Jay H.
• Assistant Examiner(s): Heitbrink, Timothy W.
• Application Number:
  US06/533,192
• Time in Patent Office: 879 Days
• Field of Search: 425/84, 425/116, 425/117, 425/121, 425/470, 249/81, 249/91, 249/113, 249/126, 249/133, 249/160, 83/915.5, 264/78, 264/158, 134/12, 203/DIG. 16, 203/60, 118/429, 118/500, 422/99, 422/102, 435/284, 435/287, 435/300, 435/301
• US Class Current: 425/117
• CPC Class Codes: G01N 1/36 Embedding or analogous mounting of samples
• CPC Class Codes: G01N 2001/315 Basket-type carriers for tissues

Abstract

Capsules in which histological tissue samples are prepared for embedding and in which the samples are embedded include a first and a second member, at least one of the members including a frame that defines an opening. The members have complementary surfaces for establishing peripheral contact between the members around the opening. A porous material is spread across the opening so that when the members are in peripheral contact with each other, a tissue-holding region is defined inward of the porous material. The porous material has a porosity that accesses processing liquids and liquified embedding material, such as molten paraffin, but prevents stray parts of the tissue from floating free of the region. Preferably, the second members also comprise a frame defining a second opening and porous material spread across the second opening. Preferably, the capsules having openings in both members are stackable in sealing relationship with one another to provide a central passageway through which processing liquids and liquified embedding material may be passed, thereby permitting simultaneous processing of a plurality of tissue samples. Preferably, one of the frames, that has an opening covered by a porous material, is adapted to retain a protruding block of solidified embedding material having the tissue sample held outward so that the one frame may be clamped in a microtome chuck and tissue slices shaved from the protruding embedding material.

Claims

1. Apparatus for the simultaneous preparation of multiple tissue specimens for histological examination comprising

a plurality of cooperating stackable capsules, each of said capsules including a mold member and a complementary removable cover member,

said mold member including an open mold frame formed from a material that is resilient and deformable under compression, said cover member including an open cover frame formed from a material that is more rigid than said mold frame, the interior sidewalls of said mold frames and said cover frames in a stack of capsules defining a continuous fluid passageway for tissue treating fluids,

said mold member including a porous web spanning said fluid passageway, said cover member including a porous web spanning said passageway, said webs in each of said capsules being spaced apart defining therebetween a tissue-receiving cavity.

2. Apparatus in accordance with claim 1 wherein said mold frame has a recessed seat formed in its upper surface adapted to receive and support said cover frame.

3. Apparatus in accordance with claim 1 including means to compress a stack of capsules.

4. Apparatus in accordance with claim 3 including upper and lower cover plates in sealing engagement with the uppermost cover member and lowermost mold member, and means for introducing tissue-treating fluids into said fluid passageway.

5. Apparatus in accordance with claim 3 including means for introducing, successively or concurrently, a plurality of tissue-treating fluids into said fluid passageway and means for collecting spent fluids from said fluid passageway.

6. Apparatus in accordance with claim 5 including mixing means for said tissue-treating fluids, said fluids including water and a miscible dehydrating solvent and control means for controlling the solvent concentration in the water-solvent mixture.

7. Apparatus in accordance with claim 5 including means to recover solvent from the spent water-solvent mixture.

8. Apparatus in accordance with claim 1 wherein the porous web has a porosity of between about 35 and about 65 percent and a maximum pore size of about 5 microns.

9. Apparatus in accordance with claim 8 wherein the porous web is nonwoven nylon.