Aqueous solution for suspending and storing cells, especially erthrocytes

• US 4,572,899 A
• Filed: 07/06/1983
• Issued: 02/25/1986
• Est. Priority Date: 07/07/1982
• Status: Expired due to Fees

• Current Assignee: NPBI Nederlands Produktielaboratorium VOOR Bloedtransfusieapparatuur EN Infusievloeistoffen BV
• Original Assignee: Biotest-Serum-Institut GmbH
• Inventors: Walker, Wolfram H., Ganshirt, Karlheinz
• Primary Examiner(s): Cockeram, Herbert S.
• Application Number:
  US06/511,434
• Time in Patent Office: 965 Days
• Field of Search: 424/101, 435/2, 435/240, 436/8, 436/16, 436/17, 436/18
• US Class Current: 436/18
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
• CPC Class Codes: A61K 35/18 Erythrocytes haemoglobin A61K38/42
• CPC Class Codes: Y10T 436/106664 Blood serum or blood plasma standard or control
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]
• CPC Class Codes: Y10T 436/108331 Preservative, buffer, anticoagulant or diluent

Abstract

An aqueous solution for suspending and storing cells, especially erythrocytes, and containing sodium chloride, glucose or fructose, adenine, and a sugar alcohol, the sugar alcohol being sorbitol or xylitol is employed to further improve the storage time and hence the survival rate of the cells and even reduce the hemolysis rate of erythrocytes in packed red blood cells.

Claims

1. Aqueous solution for suspending and storing cells, especially erythrocytes, and containing per liter 0.5-20 g of sodium chloride, 0.5-20 g of glucose or fructose, 0.05-1 g of adenine, guanosine in up to 1.5 g, 0-5 g of at least one phosphate and 1-20 g of sorbitol or xylitol.

2. A solution according to claim 1 containing sorbitol.

3. A solution according to claim 1 wherein at least one of said phosphates is hydrogenorthophosphate or a dihydrogenorthophosphate.

4. A solution according to claim 2 comprising 4.2 g of sodium chloride, 1.14 g of dibasic sodium phosphate×

2H₂ O, 1.11 g of monobasic sodium phosphate×

H₂ O, 9.4 g of glucose monohydrate, 10.0 g of sorbitol, 0.25 g of adenine hydrochloride, and 0.4 g of guanosine per liter of water for injections.

5. A solution according to claim 1 additionally containing a colloid.

6. A composition comprising erythrocytes and the aqueous solution of claim 1.

7. A composition according to claim 6 wherein said aqueous solution contains sorbitol.

8. A process for washing erythrocytes which comprises mixing said erythrocytes with the solution of claim 1, thereafter removing at least a portion of said solution, and recovering the erythrocytes.

9. A solution according to claim 1, wherein at least one of said phosphates is dibasic sodium phosphate or monobasic sodium phosphate.