Three phase irradiation treatment process

• US 4,573,960 A
• Filed: 10/29/1984
• Issued: 03/04/1986
• Est. Priority Date: 10/29/1984
• Status: Expired due to Term

• Current Assignee: McNeilab, Inc. (Johnson & Johnson)
• Original Assignee: Extracorporeal Medical Specialties Incorporated (Johnson & Johnson)
• Inventors: Goss, Jack
• Primary Examiner(s): Skogquist, Harland S.
• Application Number:
  US06/665,834
• Time in Patent Office: 491 Days
• Field of Search: 128/1.1, 604/4, 604/5, 604/6
• US Class Current: 604/6.08
• CPC Class Codes: A61M 1/3616 Batch-type treatment
• CPC Class Codes: A61M 1/36222 Details related to the interface between cassette and machine
• CPC Class Codes: A61M 1/362223 the interface being evacuated interfaces to enhance contact
• CPC Class Codes: A61M 1/36224 with sensing means or components thereof
• CPC Class Codes: A61M 1/36225 with blood pumping means or components thereof
• CPC Class Codes: A61M 1/36226 Constructional details of cassettes, e.g. specific details on material or shape
• CPC Class Codes: A61M 1/362266 Means for adding solutions or substances to the blood
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3683 using photoactive agents
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: A61M 1/3696 with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
• CPC Class Codes: A61M 2209/082 Mounting brackets, arm supports for equipment

Abstract

In a system for altering cells such as by irradiating said cells when in contact with a photoactivatable reagent useful for the extracorporeal treatment of a patient'"'"'s blood, a three phase method for performing the operations in a safe and efficacious manner.

Claims

1. In a system for altering cells including treating the cells with a photoactivatable reagent and irradiating said cells and reagent whereby said reagent is caused to be activated and to affect said cells, a three phase method for so treating the blood of a patient comprising the steps of:

in a first phase,

collecting with a collection tubing set, whole blood from a patient having previously been given a pharmaceutically effective amount of a photoactivatable agent;

passing said whole blood into a continuous centrifuge for obtaining plasma and a leukocyte enriched portion;

storing a predetermined volume of said plasma and said leukocyte enriched portion;

returning nonleukocyte enriched portions and any excess plasma to said patient through said collection tubing set;

disconnecting said patient from said collection tubing set;

in a second phase,

passing said stored leukocyte enriched portion through an irradiation tubing set, including an irradiation chamber for photoactivating said reagent, until a predetermined level of photoactivation has been achieved; and

in a third phase,

returning said irradiated leukocyte enriched portion to said patient through a return tubing set.

2. The method of claim 1 wherein said first phase further comprises the step of infusing said whole blood collected from said patient with anticoagulation means.

3. The method of claim 2 wherein said passing said whole blood step in said first phase further comprises collecting said plasma and said leukocyte enriched portion, and said nonleukocyte enriched portion and any excess plasma into first and second containers respectively.

4. The method of claim 3 wherein said passing said leukocyte enriched portion blood through said irradiation set in said second phase further comprises returning said irradiated leukocyte enriched portion to said first container upon achievement of said predetermined photoactivation level and disconnecting said first container from said irradiation tubing set.

5. The method of claim 4 wherein said returning said irradiated leukocyte rich portion to said patient in said third phase further comprises connecting said first container to said return tubing set.

6. The method of claim 5 wherein said second phase includes the step of passing said collected nonleukocyte enriched portion and said excess plasma, if any, to an elevated container for gravity infusion back to said patient.