Method of and apparatus for dispensing liquid

• US 4,574,850 A
• Filed: 01/17/1985
• Issued: 03/11/1986
• Est. Priority Date: 01/17/1985
• Status: Expired due to Term

• Current Assignee: Dade Behring, Inc. (Siemens AG)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Davis, James E.
• Primary Examiner(s): Bell, Jr., Houston S.
• Application Number:
  US06/692,409
• Time in Patent Office: 418 Days
• Field of Search: 73/864.01, 73/864.02, 73/864.11, 73/864.12, 73/864.13, 73/864.14, 73/864.15, 73/864.16, 73/864.17, 73/864.18 , 73/864.22, 73/864.23, 73/864.25, 141/1-12, 141/18, 141/67, 141/89-92, 422/63-67, 422/100
• US Class Current: 141/9
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence

Abstract

A liquid pipetting system for aspirating and dispensing small samples of fluid and diluents. The pipetting system consists of a coaxial needle serving as the pipette tip and a metering system to control the fluids. Precise dispensing of small amounts of liquid from the inner needle is made possible by surrounding the sample liquid with a miscible diluting liquid thereby establishing a controlled interface and conveying the sample liquid into the receiving chamber with that miscible diluting liquid.

Claims

1. A liquid pipetting system for aspirating and precisely dispensing small samples of a first liquid, having an outer conduit having outlet and supply ends;

means connected to the supply end of the outer conduit for supplying a liquid thereto;

an inner conduit having inlet and supply ends and coaxially disposed in the outer conduit to define an annular space therebetween;

a sample pump means connected to the supply end of the inner conduit;

means for immersing the inlet end of the inner conduit in the first liquid; and

means for selectively actuating the sample pump means to aspirate the first liquid into the inlet end of the inner conduit;

the system characterized by a diluent pump connected to the supply end of the outer conduit for supplying a diluent liquid miscible with the first liquid thereto, and means for dispensing a portion of the aspirated first liquid by selectively activating the pumps to dispense a portion of the first liquid from the inner conduit into the diluent liquid in the annular space and simultaneously to flush the inlet end of the inner conduit with the diluent liquid.

2. A system as set forth in claim 1 wherein the outer conduit has an axis and the inner conduit is positionable along the axis.

3. A system as set forth in claim 2 wherein the inner conduit is slideably supported by protuberances in the inner wall of the outer conduit.

4. A system as set forth in claim 2 wherein the immersing means incudes means for selectively extending only the inner conduit into the first liquid for aspiration.

5. A system as set forth in claim 1 wherein the immersing means includes means for dipping the inlet ends of the conduits in the first liquid, and means to selectively activate the diluent pump to aspirate the first liquid to the annular space.

6. A system as set forth in claim 1 wherein the outer conduit is of reduced diameter at the inlet end.

7. A system as set forth in claim 1 wherein the inner conduit is of reduced diameter at the inlet end.

8. A system as set forth in claim 1 wherein both conduits are of reduced diameter at their inlet ends.

9. A method for accurately aspirating and dispensing small samples of a first liquid using a probe having inner and outer coaxially disposed conduits defining a coaxial region comprising the steps of:

aspirating the first liquid into the inner conduit;

flushing the annular space between the conduits with a second liquid miscible with the first liquid to remove the first liquid from the entire exterior of the inner conduit and establish a controlled interface between the first and second liquids;

dispensing a predetermined quantity of the first liquid from the inner conduit into the second liquid in the annular space; and

flushing the annular space with a predetermined quantity of the second liquid thereby precisely dispensing the first liquid.

10. A method set forth in claim 9 which includes the additional step of contacting the tip of the outer probe with a surface to remove excess liquid prior to dispensing and flushing;

and

initially aspirating the first liquid into the annular space as well as the inner conduit.

11. A method as set forth in claim 9 wherein the inner conduit alone is extended axially for immersion in the first liquid prior to aspiration, and

withdrawn into the outer conduit prior to the initial flushing.

12. A method as set forth in claim 11 which includes the additional step of contacting the tip of the probe with a surface to remove excess liquid prior to dispensing and flushing.