Acidic heteropolysaccharide AM-2

• US 4,575,551 A
• Filed: 10/25/1983
• Issued: 03/11/1986
• Est. Priority Date: 05/02/1983
• Status: Expired due to Fees

• Current Assignee: Nakano Vinegar Co., Ltd.
• Original Assignee: Nakano Vinegar Co., Ltd.
• Inventors: Tayama, Kenji, Fujiyama, Seiichi, Minakami, Hiroyuki, Masai, Hiroshi
• Primary Examiner(s): BROWN, JOHNNIE
• Application Number:
  US06/545,270
• Time in Patent Office: 868 Days
• Field of Search: 536/1.1, 536/114, 536/123
• US Class Current: 536/123
• CPC Class Codes: A23L 29/269 of microbial origin, e.g. xanthan or dextran
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12R 2001/02 Acetobacter
• CPC Class Codes: Y10S 435/823 Acetobacter

Abstract

Novel highly viscous acidic heterpolysaccharide AM-2 is produced from a strain of genus Acetobacter isolated from vinegar mash, and is useful as an adhesive, coating agent, drilling mud additive, enhanced oil recovering agent, etc., as well as a material useful for the production of foods, drugs and cosmetics because of its safety and high viscosity.

Claims

1. Acidic heteropolysaccharide AM-2 having the following physiochemical characteristics:

(1) composition

composed of glucose, rhamnose, mannose, glucuronic acid and acetyl group as main components, the molar ratio of glucose;

rhamnose;

mannose;

glucuronic acid being 4;

0.9˜

1.1;

0.9˜

1.1;

0.9˜

1.1 and the acetyl group content is 4 to 8%;

(2) infrared spectrum

the infrared spectrum is as shown in FIG. 1;

(3) color reaction

Anthrone reaction;

positive

Carbazole reaction;

positive

Elson-Morgan'"'"'s reaction;

negative

Iodine reaction;

positive;

(4) solubility in solvents

soluble in water, and insoluble in ethanol, ether, acetone;

(5) color and appearance

the purified polysaccharide is white, cottony or fibrous;

(6) viscosity

the aqueous solution is colorless, transparent and viscous, and the viscosity of 1% solution is 1200 to 2000 cps as determined with a torque measuring viscometer at 25°

C. and a rate of 30 rpm;

(7) elemental analysis;

C;

39.9±

1%;

H;

6.2±

1%;

N;

0%;

ash;

3.0±

1.0%;

(8) Specific rotation

[α

]_D²⁷ =0˜

+20

(C=0.33 in the aqueous solution);

(9) molecular weight

about 10⁵ or above;

(10) melting point

the polysaccharide turns dark brown at 190°

C. and decomposes at 250°

C.; and

(11) nuclear magnetic resonance spectrum

the ¹³ C-nuclear magnetic resonance spectrum is as shown in FIG. 7.