Method and apparatus for measuring density profiles in microscopic tube flow

• US 4,576,477 A
• Filed: 06/22/1982
• Issued: 03/18/1986
• Est. Priority Date: 06/22/1982
• Status: Expired due to Fees

• Current Assignee: The United States of America As Represented By The Secretary of The Department of Health and
• Original Assignee: United States Department Of Health And Human Services
• Inventors: Strul, Bruno, Holliger, Christoph, Corbet, Aaron B.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US06/390,876
• Time in Patent Office: 1,365 Days
• Field of Search: 356/39, 356/40, 356/318, 356/335, 356/244, 356/246, 356/442, 250/576
• US Class Current: 356/39
• CPC Class Codes: G01N 15/0227 using imaging; using holography
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/5957 using an image detector type detector, e.g. CCD

Abstract

Particle distribution in a fluid flowing through a microscopic tube is achieved by flowing the fluid vertically-downward through a transparent capillary tube and passing light through the flowing fluid. A linear array of photodiodes responds to light passing through the fluid by registering a series of signals representing the linear projection of particles passing through the plane defined by the light source and the photodiode array. The fluid is supplied from a reservoir which can be selectively pressurized by a gas to control the egress flow rate of fluid. A syringe serves as the egress path from the reservoir and a rotatable stirring rod is disposed in the syringe to stir the egressing fluid. The syringe feeds a disposable assembly which includes a hypodermic needle feeding the capillary tube which is mounted between a microscope slide and cover in the path of the sensing light.

Claims

1. Apparatus for use in determining the density profile of a flowing concentrated suspension test fluid, said apparatus comprising:

a transparent capillary tube;

means for flowing said concentrated suspsension test fluid vertically downward through said capillary tube;

light microscope means for transmitting light through said capillary tube and said flowing concentrated suspension test fluid in a horizontal direction perpendicular to the flow direction of said concentrated suspension test fluid, said microscope means comprising an array of photodiodes and means for focusing on said array an image of the concentrated suspension test fluid flowing through said capillary tube and said transmitted light;

wherein said array of photodiodes is a horizontal linear array which extends to a greater length than the horizontal dimension of said test fluid image focused on said array; and

data acquisition and display means responsive to said photodiodes for displaying density profile information for said concentrated suspension test fluid.

2. The apparatus according to claim 1, wherein said capillary tube is made of glass.

3. The apparatus according to claims 1 or 2, wherein said means for flowing comprises:

a reservoir for said test fluid;

a hypodermic needle for delivering said test fluid to said capillary tube; and

syringe means for delivering said test fluid from said reservoir to said hypodermic needle.

4. The apparatus according to claim 3, wherein said syringe means includes an elongated syringe extending through a wall of said reservoir, and wherein said means for flowing further comprises:

an elongated glass stirring rod having a longitudinal axis and disposed in said reservoir and extending longitudinally into said syringe; and

motor means for rotating said stirring rod about said longitudinal axis.

5. The apparatus according to claim 4, wherein said means for flowing further includes:

a test fluid supply port defined in said reservoir for delivering said test fluid into said reservoir; and

a gas supply port defined in said reservoir for delivering pressurized gas into said reservoir to control the flow rate of test fluid through said syringe.

6. The apparatus according to claim 5, wherein said capillary tube and said hypodermic needle are secured together by epoxy in a disposable unit.

7. The apparatus according to claim 5, further comprising:

a transparent planar microscope slide;

a transparent slide cover; and

means securing said capillary tube to said glass slide between said slide and said cover.

8. The apparatus according to claim 7, further comprising immersion oil disposed between said slide and said cover to surround the capillary tube portion between the slide and cover, wherein the light passing through said capillary tube and flowing fluid passes through said slide and said cover.

9. The apparatus according to claim 1, wherein said means for flowing includes:

a reservoir for said test fluid;

a test fluid supply port defined in said reservoir; and

a gas supply port for delivering pressurized gas into said reservoir to control the flow rate of test fluid from said reservoir.

10. The apparatus according to claim 1, further comprising:

a transparent planar microscope slide;

a transparent slide cover; and

means securing said capillary tube to said glass slide between said slide and said cover.

11. The apparatus according to claim 10, further comprising immersion oil disposed between said slide and said cover to surround the capillary tube portion between the slide and cover, wherein the light passing through said capillary tube and flowing fluid passes through said slide and said cover.

12. The apparatus according to claims 1 or 11, wherein said microscope means includes a xenon arc light source.

13. The apparatus according to claims 1 or 12, wherein said microscope means includes a periodic strobe light source.

14. The apparatus according to claim 1, wherein said means for focussing comprises a projection lens disposed between said capillary tube and said array of photodiodes.

15. The apparatus according to claim 1, wherein said photodiodes are successively spaced by equal distances with the density on the order of 1024 diodes to the inch.

16. The apparatus according to claim 1, further comprising:

clock means for providing a periodic time synchronizing signal; and

signal sample and hold means responsive to said time synchronizing signal for sequentially delivering sampling and integrating signals from said photodiodes.

17. The apparatus according to claim 1, wherein said test fluid is whole blood.

18. A method for use in determining the density profile of a flowing concentrated suspension test fluid, said method comprising the steps of:

flowing the concentrated suspension test fluid vertically-downward through a transparent capillary tube;

passing light through the capillary tube and the flowing concentrated suspension test fluid in a direction perpendicular to the concentrated suspension test fluid flow through said capillary tube;

imaging the concentrated suspension test fluid which flows through the capillary tube and through the passing light onto a horizontally extending linear array of photodiodes having a greater horizontal array length than the horizontal dimension of the image of the concentrated suspension test fluid at said array; and

acquiring and displaying density profile information for said concentrated suspension test fluid from said array of photodiodes.

19. The method according to claim 18, wherein the step of flowing the test fluid includes the steps of:

forcing the test fluid out from a reservoir by delivering gas under pressure to the reservoir; and

stirring the test fluid in the reservoir before it is forced therefrom.

20. The method according to claims 18 or 19, wherein the step of passing light includes periodically pulsing a light source to provide a periodically interrupted beam of light which is passed through said capillary tube.

21. The method according to claim 18, further comprising the step of sequentially sampling and integrating signals provided by said photodiodes.

22. The method according to claim 18, wherein said test fluid is whole blood.

23. The method according to claim 18 wherein the step of passing light includes the steps of:

emitting a series of light pulses having pulse widths on the order of a few microseconds toward said capillary tube; and

focusing the light pulses passing through said capillary tube onto said array of photodiodes.