Analytical element

• US 4,576,793 A
• Filed: 08/29/1983
• Issued: 03/18/1986
• Est. Priority Date: 09/07/1982
• Status: Expired due to Term

• Current Assignee: Konishiroku Photo Industry Co., Ltd.
• Original Assignee: Konishiroku Photo Industry Co., Ltd.
• Inventors: Koyama, Mikio, Okaniwa, Kenichiro, Saruhashi, Masakuni
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Delahunty, C. M.
• Application Number:
  US06/527,681
• Time in Patent Office: 932 Days
• Field of Search: 422/56, 422/57, 422/58, 422/60, 422/69, 422/86, 422/55, 436/169, 436/170, 435/805
• US Class Current: 422/423
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10T 436/172307 Cyanide or isocyanide

Abstract

There is disclosed an analytical element comprising a support; a reagent layer which is positioned on one side of the support and contains a reagent reactive with a component in a fluid sample; and a porous spreading layer which is positioned on the reagent layer on the opposite side to that of the support for permitting the component in the fluid sample to permeate into the reagent layer; wherein at least one layer contains 5% by weight or more of a polymer formed by, (i) a copolymerizable ethylenically unsaturated acid with (ii) a copolymerizable ethylenically unsaturated monomer.

The analytical element according to the present invention can determine a component in a fluid sample quantitatively.

Claims

1. An analytical element comprising a support layer having an upper side and a lower side, a reagent layer which is positioned on one of said sides of said support and contains a reagent reactive with a component in a fluid sample, and a porous spreading layer which is positioned on the reagent layer on the opposite side to said support for permitting the component in said fluid sample to permeate into said reagent layer;

wherein at least one of said layers contains at least 5% by weight of a copolymer formed by the polymerization of (i) at least 45% by weight of a copolymerizable ethylenically unsaturated acid with (ii) a copolymerizable ethylenically unsaturated monomer other than said copolymerizable ethylenically unsaturated acid.

2. An analytical element according to claim 1, wherein said copolymerizable ethylenically unsaturated acid is selected from the group consisting of the following formulas [I] and [II]:

##STR8## wherein R₁ represents a carboxyl group, a sulfo group or a group having a carboxyl group or a sulfo group, R₂ represents a hydrogen atom or an alkyl group and R₃ represents a hydrogen atom or an alkoxycarbonyl group, and ##STR9## wherein R₄ represents a hydrogen atom or a methyl group, R₅ represents an aliphatic hydrocarbon group having 2 to 10 carbon atoms or ##STR10## where R₇ is an aliphatic hydrocarbon group having 2 to 6 carbon atoms and m is an integer of 1 to 5

and R₆ represents a hydrogen atom.

3. An analytical element according to claim 1, wherein said copolymer is present in said reagent layer.

4. An analytical element according to claim 1, wherein said copolymerizable ethylenically unsaturated monomer (ii) is selected from the group consisting of an ethylenically unsaturated nitrile, a styrene, an ethylenically unsaturated acid ester, an ethylenically unsaturated acid amide, a conjugated diene, a vinyl heterocyclic compound, a halo-substituted ethylene and a cross-linkable monomer.

5. An analytical element according to claim 4, wherein said ethylenically unsaturated monomer (ii) is an ethylenically unsaturated nitrile represented by the following formula [III]:

##STR11## wherein R₈ and R₉ each represent a hydrogen atom or a halogen atom, R₁₀ represents a halogen atom, an alkyl group, an alkoxy group, an acyloxy group, an aryl group, a cyano group or a carbamoyl group.

6. An analytical element according to claim 4, wherein said ethylenically unsaturated monomer (ii) is a styrene represented by the following formula [IV]:

##STR12## wherein R₁₁ represents a hydrogen atom, a halogen atom or an alkyl group, R₁₂ represents a hydrogen atom, a halogen atom, an allkyl group, an alkoxy group, a cycloalkyl group, an alkoxycarbonyl group or an acryloxyalkyl group, and n is an integer of 1 to 5.

7. An analytical element according to claim 4, wherein said ethylenically unsaturated monomer (ii) is an ethylenically unsaturated acid ester represented by the following formula [V]:

##STR13## wherein R₁₃ represents a hydrogen atom or a methyl group, and R₁₄ represents an alkyl group or a phenyl group.

8. An analytical element according to claim 4, wherein said ethylenically unsaturated monomer (ii) is a conjugated diene represented by the following formula [VI]:

##STR14## wherein R₁₅ through R₂₀ individually represent a hydrogen atom, a halogen atom, an alkyl group, an aryl group, a cyano group or a group

space="preserve" listing-type="equation">--COOR.sub.21

where R₂₁ represents an alkyl group.

9. An analytical element according to claim 1, wherein said copolymer is copoly(2-acrylamide-2-methylethane sulfonic acid--n-butyl acrylate.

10. An analytical element according to claim 9, wherein the weight ratio of sulfonic acid to n-butyl acrylate in said copolymer is 4:

1.

11. An analytical element according to claim 10, wherein said copolymer is present in said reagent layer.

12. An analytical element according to claim 1, wherein said copolymer is copoly(2-acrylamide-2-methylpropane sulfonic acid--butyl acrylate.

13. An analytical element according to claim 12, wherein the weight ratio of sulfonic acid to butyl acrylate in said copolymer is 1:

1.

14. An analytical element according to claim 13, wherein said copolymer is present in said reagent layer.

15. An analytical element according to claim 1, wherein said copolymer is copoly(2-acrylamide-2-methylpropane sulfonic acid--acrylamide.

16. An analytical element according to claim 15, wherein the weight ratio of sulfonic acid to acrylamide is said copolymer is 1:

1.

17. An analytical element according to claim 16, wherein said copolymer is present in said reagent layer.