Centrifugal tissue processor

• US 4,576,796 A
• Filed: 01/18/1984
• Issued: 03/18/1986
• Est. Priority Date: 01/18/1984
• Status: Expired due to Term

• Current Assignee: Pelam, Inc.
• Original Assignee: Pelam, Inc.
• Inventors: McCormick, James B.
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/572,168
• Time in Patent Office: 790 Days
• Field of Search: 422/63, 422/64, 422/72, 422/99, 422/100, 422/102, 427/2, 427/4, 427/24, 118/52, 494/20, 435/312
• US Class Current: 422/536
• CPC Class Codes: G01N 1/36 Embedding or analogous mounting of samples

Abstract

Tissue samples are prepared for embedding and then embedded within wells of an intermittently rotatable rotor. After each successive liquid is supplied to the sample wells, the rotor is spun in order to slough off liquid from the samples. The sloughed liquid is collected by an outer shroud. The shroud is preferably made fluid tight so that the interior region of the shroud can be communicated to vacuum to force embedding material into the interstices of the tissue samples.

Claims

1. Apparatus for processing and embedding tissue samples comprising

rotor means having a rotational axis and a plurality of well means outward of its rotational axis for holding tissue samples,

means to successively supply processing liquids and liquid embedding material to said well means,

means to intermittently rotate said rotor means at a sufficient speed to slough liquid from said well means, and

well cover means which retain said tissue samples in said well means while accessing processing liquids and embedding liquids to said well means, said well cover means comprising a peripheral frame defining a fluid passage opening and a porous mesh spanning said opening.

2. Apparatus according to claim 1 including means to heat said rotor means.

3. Apparatus in accordance with claim 1 wherein said well means are shallow trays pivotably mounted on said rotor and adapted to pivot from a horizontal position when said rotor is at rest to a vertical position when said rotor is rotated.

4. Apparatus according to claim 1 wherein said rotor has a central well means and channels means extending radially therefrom to said well means, whereby said well means is supplied with processing liquids and liquified embedding material from said central well means through said channel means.

5. Apparatus according to claim 4 wherein said channel means extend radially outward from said outward well means providing a liquid flow pathway for liquid spun from said well means.

6. Apparatus according to claim 1 wherein said means to successively supply processing and liquified embedding material to said well means comprises a plurality of reservoirs containing processing liquids and liquified embedding material and means to individually communicate said reservoirs to said well means.

7. Apparatus according to claim 6 including automatic programmable control means for successively communicating said reservoirs to said well means and intermittently actuating said rotor means to spin liquids therefrom, according to a processing cycle.

8. Apparatus according to claim 1 including a shroud surrounding said rotor for collecting processing liquids that overflow from said well means and which are spun from said well means.

9. Apparatus according to claim 8, said shroud having a lower sump opening.

10. Apparatus according to claim 8 having an open upper end and shroud cover means for closing off said open upper end.

11. Apparatus according to claim 8, said rotor having a central well means and channel means extending radially therefrom to said outward well means, said rotor cover having a central inlet conduit that leads externally, whereby liquid may be introduced to said central well means through said inlet conduit and flow through said radial channel means to said outward well means.

12. Apparatus according to claim 8 including sealing means between said cover and said shroud and means to communicate the interior of said shroud to vacuum for vacuum-embedding tissue samples within said well means.

13. Apparatus for processing and embedding tissue samples comprising

rotor means having a rotational axis and a plurality of well means outward of its rotational axis for holding tissue samples,

means to successively supply processing liquids and liquified embedding material to said well means,

means to intermittently rotate said rotor means at a sufficient speed to slough liquid from said well means, and

well cover means which retain said tissue samples in said well means while accessing processing liquids and embedding liquids to said well means, said well cover means comprising a peripheral frame defining a fluid passage opening and a porous mesh spanning said opening below an upper surface of said peripheral frame providing a region for embedding material above said mesh, whereby when liquified embedding material solidifies, said mesh is embedded therein and said cover is attached to said solidified embedding material.

14. Apparatus in accordance with claim 13 wherein said well cover means is detachable from said rotor and adapted to be inserted into a microtome chuck, whereby said solidified embedding material may be positioned for microtome slicing.