Polylactic-polyglycolic acids combined with an acidic phospholipid-lysozyme complex for healing osseous tissue

• US 4,578,384 A
• Filed: 02/15/1984
• Issued: 03/25/1986
• Est. Priority Date: 02/15/1984
• Status: Expired due to Fees

• Current Assignee: United States Of America As Represented By The Secretary Of The Army (Government of the United States of America)
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Hollinger, Jeffrey O.
• Primary Examiner(s): Phillips, Delbert R.
• Assistant Examiner(s): Moezie, F. T.
• Application Number:
  US06/580,496
• Time in Patent Office: 769 Days
• Field of Search: 260/112.5 R, 424/22, 424/177
• US Class Current: 514/16.7
• CPC Class Codes: A61L 2300/22 Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
• CPC Class Codes: A61L 2300/254 Enzymes, proenzymes
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2300/604 Biodegradation
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

A novel material consisting of a combination of a proteolipid and biodegradable, biocompatible copolymer has been developed for improving and accelerating the healing of osseous tissue. The proteolipid was prepared by combining muco-peptide-N-acetyl-muramoylhydrolase with phosphatidyl inositol, 4,5-diphosphate and the copolymer consists essentially of a 50:50 poly(L)(-)lactide co-glycolide.

Claims

1. A method for improving and promoting the healing of osseous tissue in an animal which comprises implanting at the site of the broken osseous tissue an effective amount of a biodegradable copolymer-proteolipid material, said material consisting essentially of from 1 to 5 weight percent of the mucopeptide-N-acetylmuramoylhydrolase:

phosphatidyl inositol 4,5-diphosphate, incorporated into from 95 to 99 weight percent of the biodegradable copolymer, 50;

50 poly(L)(-)lactide co-glycolide.

2. The method of claim 1 wherein the amount of mucopeptide-N-acetylmuramoylhydrolase:

phosphatidyl inositol 4,5-diphosphate is about 1 percent by weight of said copolymer.

3. A pharmaceutical composition useful in improving and promoting the healing of broken osseous tissue comprising an effective amount of from 1-5 weight percent of mucopeptide-N-acetylmuramoylhydrolase:

phosphatidyl inositol 4,5-diphosphate incorporated into from 95-99 weight percent of the biodegradable copolymer of 50;

50 poly(L)(-)lactide co-glycolide.

4. The composition of claim 3 wherein the amount of mucopeptide-N-acetylmuramoylhydrolase:

phosphatidyl inositol 4,5-diphosphate is about 1 percent by weight of said copolymer.