Electrochemical sensor having an immobilized enzyme membrane
First Claim
1. An electrochemical sensor which comprises a vessel formed with a liquid-junction at an end face of the vessel, a resilient ion selective membrane disposed at the liquid-junction, and an immobilized enzyme membrane arranged to cover said ion selective membrane;
- a recess of a predetermined depth being formed in the end face of said vessel and the resilient ion selective membrane having a thickness larger than the depth of said recess;
said ion selective membrane being secured to said recess and pressed therein by the immobilized enzyme membrane.
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Accused Products
Abstract
A recess of a predetermined thickness is formed in the bottom of a cylindrical vessel and a hole for the liquid-junction is formed at the center of the recess, to which is joined a resilient NH4+ selective membrane. Since the NH4+ selective membrane has a thickness larger by about 0.1 mm than the depth of the recess, the NH4+ selective membrane is protruded out of the bottom of the vessel by so much. A polyester woven fabric on which urease is immobilized is attached to the vessel so as to cover the NH4+ selective membrane. Since the immobilized enzyme membrane is applied with an appropriate tension by the slightly protruded ion selective membrane, bondability between the two membranes can be improved.
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Citations
12 Claims
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1. An electrochemical sensor which comprises a vessel formed with a liquid-junction at an end face of the vessel, a resilient ion selective membrane disposed at the liquid-junction, and an immobilized enzyme membrane arranged to cover said ion selective membrane;
- a recess of a predetermined depth being formed in the end face of said vessel and the resilient ion selective membrane having a thickness larger than the depth of said recess;
said ion selective membrane being secured to said recess and pressed therein by the immobilized enzyme membrane. - View Dependent Claims (2, 3, 4)
- a recess of a predetermined depth being formed in the end face of said vessel and the resilient ion selective membrane having a thickness larger than the depth of said recess;
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5. An electrochemical sensor comprising a vessel means having a liquid-junction opened at the bottom end face of the vessel means;
- a recess formed in the end face of said vessel means and in communication with said liquid-junction, a resilient ion selective membrane arranged partially in said recess, the membrane having substantially the same plane size as that of said recess and a thickness larger than the depth of said recess, and an immobilized enzyme membrane less swollen to water covering said ion selective member to urge said ion selective membrane against said recess.
- View Dependent Claims (6, 7, 8)
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9. An electrochemical sensor comprising a cylindrical vessel containing an electrolyte and having an opening at one end face forming a liquid-junction, a recess formed in the end face and in communication with said liquid-junction;
- a resilient ion selective membrane located partially within said recess, and an immobilized enzyme membrane arranged to cover the end face of the vessel and to press the ion selective membrane against the recess;
said ion selective membrane having a thickness that is greater than the depth of the recess so that a surface of the ion selective membrane protrudes from the end face of the vessel and said immobilized enzyme membrane comprising a woven fabric which intimately contacts the protruding surface of the ion selective membrane whereby good bondability is provided between the ion selective membrane and the immobilized enzyme membrane. - View Dependent Claims (10, 11, 12)
- a resilient ion selective membrane located partially within said recess, and an immobilized enzyme membrane arranged to cover the end face of the vessel and to press the ion selective membrane against the recess;
Specification