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Element for electrophoresis

  • US 4,579,783 A
  • Filed: 05/18/1984
  • Issued: 04/01/1986
  • Est. Priority Date: 05/19/1983
  • Status: Expired due to Term
First Claim
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1. An element for electrophoresis comprising the following three-layer structure laminated in the order:

  • (I) a support layer;

    (II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of;

    (1) a repeating unit having the formula (1);

    ##STR22## in which R11 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

    Q1 is --COO--, --CON(R11)--, or an arylene group containing 6-10 carbons atoms;

    L1 is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R11)-- and containing 3-15 carbon atoms, or a divalent group containing at least one linkage selected from the group consisting of --O--, --N(R11)--, --CO--, --SO--, --SO2 --, --SO3 --, --SO2 N(R11)--, --N(R11)CON(R11)-- and --N(R11)COO--, and containing 1-12 carbon atoms, in which R11 has the same meaning as defined above;

    R12 is --CH═

    CH2 or --CH2 CH2 X1, in which X1 is a substituent replaceable with a nucleophilic troup or releasable in the form of HX1 by a base;

    A1 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and

    x1, y1 and z1 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively;

    (2) a repeating unit having the formula (2);

    ##STR23## in which R21 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

    R22 is --CH═

    CH2 or --CH2 CH2 X2, in which X2 is a substituent replaceable with a nucleophilic group or releasable in the form of HX2 by a base;

    L2 is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ2 --, and --COZ2 R23 --, in which R23 is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z2 is the oxygen atom or NH;

    A2 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and

    x2, y2 and z2 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and

    (3) a repeating unit having the formula (3);

    ##STR24## in which R31 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

    L3 is a divalent linkage group containing 1-20 carbon atoms;

    X3 is an active ester;

    A3 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions;

    x3, y3 and z3 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and

    m is 0 or 1; and

    (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, a water-soluble polymer, agarose and a compound containing at least one carbamoyl group serving as modifier.

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