Element for electrophoresis

• US 4,579,783 A
• Filed: 05/18/1984
• Issued: 04/01/1986
• Est. Priority Date: 05/19/1983
• Status: Expired due to Term

• Current Assignee: Director of The Finance Division Minister's Secretariat Science and Technology Agency
• Original Assignee: Director of The Finance Division Minister's Secretariat Science and Technology Agency
• Inventors: Shiraishi, Hisashi, Ogawa, Masashi, Ikeda, Teppei
• Primary Examiner(s): Buffalow, Edith
• Application Number:
  US06/611,594
• Time in Patent Office: 683 Days
• Field of Search: 204/299 R, 204/180 G, 526/287, 428/483, 428/475.2, 524/521
• US Class Current: 204/616
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10T 428/31736 Next to polyester
• CPC Class Codes: Y10T 428/31797 Next to addition polymer from unsaturated monomers

Abstract

An element for electrophoresis comprising the following three-layer structure laminated in the order:

(I) a support layer;

(II) an adhesive layer comprising a copolymer having at least one specifically selected repeating unit; and

(III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, and a compound containing at least one carbamoyl group (modifier).

Claims

1. An element for electrophoresis comprising the following three-layer structure laminated in the order:

(I) a support layer;

(II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of;

(1) a repeating unit having the formula (1);

##STR22## in which R¹¹ is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

Q¹ is --COO--, --CON(R¹¹)--, or an arylene group containing 6-10 carbons atoms;

L¹ is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R¹¹)-- and containing 3-15 carbon atoms, or a divalent group containing at least one linkage selected from the group consisting of --O--, --N(R¹¹)--, --CO--, --SO--, --SO₂ --, --SO₃ --, --SO₂ N(R¹¹)--, --N(R¹¹)CON(R¹¹)-- and --N(R¹¹)COO--, and containing 1-12 carbon atoms, in which R¹¹ has the same meaning as defined above;

R¹² is --CH═

CH₂ or --CH₂ CH₂ X¹, in which X¹ is a substituent replaceable with a nucleophilic troup or releasable in the form of HX¹ by a base;

A¹ is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and

x¹, y¹ and z¹ all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively;

(2) a repeating unit having the formula (2);

##STR23## in which R²¹ is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

R²² is --CH═

CH₂ or --CH₂ CH₂ X², in which X² is a substituent replaceable with a nucleophilic group or releasable in the form of HX² by a base;

L² is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ² --, and --COZ² R²³ --, in which R²³ is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z² is the oxygen atom or NH;

A² is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and

x², y² and z² all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and

(3) a repeating unit having the formula (3);

##STR24## in which R³¹ is the hydrogen atom or an alkyl group containing 1-6 carbon atoms;

L³ is a divalent linkage group containing 1-20 carbon atoms;

X³ is an active ester;

A³ is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions;

x³, y³ and z³ all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and

m is 0 or 1; and

(III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, a water-soluble polymer, agarose and a compound containing at least one carbamoyl group serving as modifier.

2. The element for electrophoresis as claimed in claim 1, in which said compound serving as modifier is urea or formamide.

3. The element for electrophoresis as claimed in claim 1, in which said support layer is made of a plastic material sheet.

4. The element for electrophoresis as claimed in claim 3, in which the plastic material sheet is a polyethylene terephthalate sheet.

5. The element for electrophoresis as claimed in claim 1, in which the adhesive layer consists essentially of a polymer having the formula (1).

6. The element for electrophoresis as claimed in claim 1 in which said water-soluble polymer is contained in the amount of 2 to 100 wt.% based on the polyacrylamide solid and said agarose is contained in the aqueous polyacrylamide gel in an amount of 0.2 to 2 wt/v %.