Hematology control

• US 4,579,824 A
• Filed: 05/18/1983
• Issued: 04/01/1986
• Est. Priority Date: 05/18/1983
• Status: Expired due to Fees

• Current Assignee: Louderback, Allan L., Paul Szatkowski
• Original Assignee: Louderback, Allan L., Paul Szatkowski
• Inventors: Louderback, Allan L., Szatkowski, Paul
• Primary Examiner(s): Gron, Teddy S.
• Assistant Examiner(s): Kilby, Catherine S.
• Application Number:
  US06/495,629
• Time in Patent Office: 1,049 Days
• Field of Search: 436/10, 436/63, 436/178, 252/408.1
• US Class Current: 436/10
• CPC Class Codes: G01N 2496/05 containing blood cells or plasma
• CPC Class Codes: G01N 33/96 involving blood or serum control standard
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/101666 Particle count or volume standard or control [e.g., platelet count standards, etc.]
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]

Abstract

A hematology control is disclosed which is useful for clinical hematology procedures and, in particular, mean corpuscular volume (MCV) determinations. The hematology control comprises an aqueous suspension of red blood cells in which varying levels of MCV are provided by applying to the red cell component, after light treatment with aldehyde, an osmotic pressure that is directly proportional to the desired red blood cell size.

Claims

1. A hematology control for mean corpuscular volume determinations comprising an aqueous suspension of mammalian red blood cells in which said red blood cells have been treated to provide a predetermined mean corpuscular volume by contacting with an aqueous aldehyde solution in 0.9% saline in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18°

to about 28°

C., whereby said red blood cells retain their plasticity for up to about 5 days and then washing said red cells while still retaining said plasticity with Alsever'"'"'s Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to provide an osmotic pressure on the cells which is directly proportional to the cell size required for the predetermined mean corpuscular volume.

2. The hematology control of claim 1 in which the predetermined mean corpuscular volume ranges from about 75 to about 95μ

³ and the osmotic pressure ranges from about 115 to about 275 milliosmols.

3. The hematology control of claim 1 in which the mammalian red blood cells are human red blood cells.

4. The hematology control of claim 3 including simulated human white blood cells comprising avian red blood cells which have been stringently fixed with dilute aldehyde solution whereby said cells become hard.

5. The hematology control of claim 1 in which the red blood cells are suspended in 0.9% saline solution during said contacting with aldehyde.

6. The hematology control of claim 1 in which the aldehyde is formaldehyde.

7. The hematology control of claim 4 in which the concentration of the human red blood cells ranges from about 3×

10⁶ to about 5×

10⁶ /mm³ and in which the concentration of the avian red blood cells ranges from about 5×

10³ to about 15×

10³ /mm³.

13. The hematology control of claim 1 including platelets which have been treated with aldehyde and modified Alsever'"'"'s Solution in the same manner as the red blood cells.

14. The hematology control of claim 3 including human platelets which have been treated with aldehyde and modified Alsever'"'"'s Solution in the same manner as the red blood cells.

8. A method of making a hematology control having a predetermined mean corpuscular volume comprising treating mammalian red blood cells with an aqueous solution of aldehyde in 0.9% saline solution in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18°

to about 28°

C., whereby said red cells retain their plasticity for up to about 5 days, adjusting the mean corpuscular volume to any desired level within a range of from about 75 to about 95μ

³ by thoroughly washing the red blood cells while still retaining said plasticity in Alsever'"'"'s Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to thereby provide an osmotic pressure on the red blood cells of from about 115 to about 275 milliosmols, and thereafter placing the red blood cells in aqueous suspension.

9. The method of claim 8 in which the mammalian red blood cells are human red blood cells.

10. The method of claim 8 in which the aldehyde is formaldehyde.

11. The method of claim 8 in which the pH is about 7.2.

12. The method of claim 8 in which the modified Alsever'"'"'s Solution contains a sugar component consisting of sucrose having a concentration of about 10 gm per liter.