Hematology control
First Claim
1. A hematology control for mean corpuscular volume determinations comprising an aqueous suspension of mammalian red blood cells in which said red blood cells have been treated to provide a predetermined mean corpuscular volume by contacting with an aqueous aldehyde solution in 0.9% saline in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18°
- to about 28°
C., whereby said red blood cells retain their plasticity for up to about 5 days and then washing said red cells while still retaining said plasticity with Alsever'"'"'s Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to provide an osmotic pressure on the cells which is directly proportional to the cell size required for the predetermined mean corpuscular volume.
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Abstract
A hematology control is disclosed which is useful for clinical hematology procedures and, in particular, mean corpuscular volume (MCV) determinations. The hematology control comprises an aqueous suspension of red blood cells in which varying levels of MCV are provided by applying to the red cell component, after light treatment with aldehyde, an osmotic pressure that is directly proportional to the desired red blood cell size.
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Citations
14 Claims
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1. A hematology control for mean corpuscular volume determinations comprising an aqueous suspension of mammalian red blood cells in which said red blood cells have been treated to provide a predetermined mean corpuscular volume by contacting with an aqueous aldehyde solution in 0.9% saline in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18°
- to about 28°
C., whereby said red blood cells retain their plasticity for up to about 5 days and then washing said red cells while still retaining said plasticity with Alsever'"'"'s Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to provide an osmotic pressure on the cells which is directly proportional to the cell size required for the predetermined mean corpuscular volume. - View Dependent Claims (2, 3, 4, 5, 6, 7, 13, 14)
- to about 28°
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8. A method of making a hematology control having a predetermined mean corpuscular volume comprising treating mammalian red blood cells with an aqueous solution of aldehyde in 0.9% saline solution in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18°
- to about 28°
C., whereby said red cells retain their plasticity for up to about 5 days, adjusting the mean corpuscular volume to any desired level within a range of from about 75 to about 95μ
3 by thoroughly washing the red blood cells while still retaining said plasticity in Alsever'"'"'s Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to thereby provide an osmotic pressure on the red blood cells of from about 115 to about 275 milliosmols, and thereafter placing the red blood cells in aqueous suspension. - View Dependent Claims (9, 10, 11, 12)
- to about 28°
Specification