Process and apparatus for electrically desorbing components selectively sorbed on an electrolytically conducting barrier

• US 4,584,075 A
• Filed: 11/26/1984
• Issued: 04/22/1986
• Est. Priority Date: 11/26/1984
• Status: Expired due to Fees

• Current Assignee: Ionics Incorporated
• Original Assignee: Ionics Incorporated
• Inventors: Haber, Edgar, Goldstein, Arthur L., Mandle, Robert J.
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): Chapman, Terryence
• Application Number:
  US06/675,057
• Time in Patent Office: 512 Days
• Field of Search: 204/180 P, 204/263, 204/296, 204/301
• US Class Current: 204/522
• CPC Class Codes: B01D 15/00 Separating processes involving the treatment of liquids with solid sorbents using liquid sorbents B01D11/00; ion exchange processes or materials, sorbent materials in general B01J, e.g. sorbents for chromatography B01J20/281; for investigating or analysing materials G01N30/00; Apparatus therefor
• CPC Class Codes: B01D 67/0093 Chemical modification
• CPC Class Codes: B01D 69/141 Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: Y10S 436/824 Immunological separation techniques

Abstract

Processes and apparatuses are described for recovering components dispersed in an aqueous solution comprising contacting said solution as a first solution with an electrically conducting barrier, diaphragm or membrane which has a high affinity for at least one of said components whereby a substantial fraction of said component is sorbed by said barrier. The barrier is subsequently contacted with a second aqueous solution and a direct electric current is passed through said barrier and second solution in a direction substantially parallel to the smallest dimension of the barrier thereby facilitating desorption of at least one of the sorbed components from the barrier into the second solution which solution is thereafter removed.

Claims

1. A method of recovering biospecifically, sorbable components dispersed in an aqueous solution comprising:

(a) contacting said solution as a first solution with an electrolytically conducting barrier having biospecific sorbing properties for at least one of said components whereby a substantial fraction of said components in said solution having biospecific binding affinity for said barrier is sorbed by said barrier;

(b) removing said first solution from contact with the said barrier;

(c) contacting said barrier with a second aqueous solution;

(d) passing a direct electric current through said barrier and said second solution in a direction approximately parallel to the smallest dimension of said barrier thereby facilitating the desorption of at least one of said components having biospecific binding affinity for the said barrier into said second solution; and

(e) removing said second solution from contact with said barrier.

2. Apparatus for recovering biospecifically, sorbable components dispersed in an aqueous solution comprising:

(a) an electrolytically conducting barrier having biospecific sorbing properties for at least one of said components;

(b) means for contacting said solution with said barrier whereby components in said solution having biospecific binding affinity for said barrier are at least in part sorbed by said barrier;

(c) means for removing said solution from contact with said barrier;

(d) means for contacting said barrier with a second aqueous solution as a receiving solution;

(e) means for passing a direct electric current through said barrier and said second solution in a direction approximately parallel to the smallest dimension of said barrier thereby to facilitate the desorption from said barrier of at least one of said components having biospecific binding affinity for said barrier into said second solution and

(f) means for removing said second solution from contact with said barrier.

3. Apparatus according to claim 2 in which said barrier has the form of a cylinder.

4. Apparatus according to claim 2 in which said barrier has the form of a spiral.

5. Apparatus according to claim 2 in which said barrier contain ligands selected from the group consisting of enzyme inhibitors, enzyme substrate analogues, coenzymes, apoenzyme polymeric substrates, enzymes, nucleic acid complementary strands, haptens, antibodies, monosaccharides, polysaccharides, lectins, small target compounds for binding proteins, binding proteins, aminobenzamidine, 5'"'"' adenosine monophosphate, S. aureus Protein A, hormones, hormone receptors, and immobilized textile dyes.

6. Apparatus according to claim 2 in which said barrier is selected from the group consisting of ion-selective membranes, sheets of hydrous gels and porous sheets having average pore sizes of less than about 10 micrometers.

7. Apparatus according to claim 2 in which said barrier is a macroreticular ion-selective membrane.

8. Apparatus according to claim 2 in which said barrier is an ion-selective membrane having ligands attached to a macroreticular surface.

9. Apparatus according to claim 2 in which said barrier is a bipolar ion selective membrane.