Fluorescence polarization immunoassay

• US 4,585,862 A
• Filed: 02/08/1984
• Issued: 04/29/1986
• Est. Priority Date: 12/11/1981
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Jolley, Michael E., Stroupe, Stephen D., Wang, Chao-Huei J.
• Primary Examiner(s): Welsh, Maurice J.
• Assistant Examiner(s): Moore, M. L.
• Application Number:
  US06/577,946
• Time in Patent Office: 811 Days
• Field of Search: 549/223, 549/225, 544/319
• US Class Current: 544/319
• CPC Class Codes: C07D 311/90 with hydrocarbon radicals, substituted by amino radicals, directly attached in position 9
• CPC Class Codes: C07D 405/12 linked by a chain containing hetero atoms as chain links
• CPC Class Codes: G01N 33/533 with fluorescent label

Abstract

This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of the specific ligand present in a sample.

Claims

1. A compound of the formula ##STR42## wherein R is a ligand-analog of an aromatic carbon-containing ligand having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has an aromatic carbon by which the ligand-analog is attached to the sulfonamidofluorescein moiety;

and biologically acceptable salts thereof.

2. A compound according to claim 1 of the formula:

##STR43## and biologically acceptable salts thereof.

3. A compound according to claim 2 wherein R is derived from a ligand selected from the group consisting of steroids, hormones, antiasthmatics, antineoplastics, antiarrhythmics, anticonvulsants, antibiotics;

antiarthritics, antidepressants and cardiac glycosides.

4. A compound according to claim 3 wherein R is derived from an antiarrhythmic.

5. A compound according to claim 4 wherein R is derived from lidocaine.

6. The compound of claim 1 having the structural formula ##STR44##

7. A compound according to claim 3 wherein R is derived from an anticonvulsant.

8. A compound according to claim 7 wherein R is derived from phenytoin.

9. A compound according to claim 8 wherein R is ##STR45##

10. A compound according to claim 7 wherein R is derived from primidone.

11. A compound according to claim 7 wherein R is ##STR46## wherein R'"'"' is alkyl having from one to four carbon atoms.

12. A compound according to claim 7 wherein R is derived from ethosuccimide.

13. A compound according to claim 12 wherein R is ##STR47## wherein n is an integer of from 0 to 3.

14. A compound of the formula ##STR48## wherein R is a ligand-analog having a molecular weight in range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog is attached to the carboamidosulfonylaminofluorescein moiety through a hydroxy oxygen or a reactive amine;

and biologically acceptable salts thereof.

15. A compound according to claim 14 of the formula ##STR49## and biologically acceptable salts thereof.

16. A compound according to claim 15 wherein R is derived from a ligand selected from the group consisting of steroids, hormones, antiasthmatics, antineoplastics, antiarrhythmics, anticonvulsants, antibiotics, antiarthritics, antidepressants and cardiac glycosides.

17. A compound according to claim 16 wherein R is derived from an anticonvulsant.

18. A compound according to claim 17 wherein R is derived from carbamazepine.

20. A compound according to claim 16 wherein R is derived from an antiarrhythmic.

21. A compound according to claim 20 wherein R is derived from procainamide.

22. A compound according to claim 20 wherein R is derived from propranolol.

19. A compound of the formula wherein R is ##STR50##

23. A compound of the formula ##STR51## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has a reactive nitrogen by which the ligand-analog is attached to the oxalylaminofluorescein moiety;

and biologically acceptable salts thereof.

24. A compound of the formula:

##STR52## and biologically acceptable salts thereof.

25. A compound according to claim 24 wherein R is derived from a ligand selected from the group consisting of steroids, hormones, antiasthmatics, antineoplastics, antiarrhythmics, anticonvulsants, antibiotics, antiarthritics, antidepressants and cardiac glycosides.

26. A compound according to claim 25 wherein R is derived from an antidepressant.

27. A compound according to claim 26 wherein R is derived from a tricyclic drug.

28. A compound according to claim 27 wherein R is derived from nortriptyline, amitriptyline, imipramine or desipramine.

29. A compound according to claim 28 wherein R is derived from an anticonvulsant.

30. A compound according to claim 29 wherein R is derived from carbamazepine.

31. A compound according to claim 30 wherein R is ##STR53##

32. A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula:

##STR54## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has an aromatic carbon by which the ligand-analog is attached to the sulfonamidofluorescein moiety;

and an antibody capable of specifically recognizing said ligand and said tracer and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.

33. A method according to claim 32 wherein the sulfonamido group bonded to the R group is also bonded to the 4- or 5-position of the fluorescein moiety.

34. A method according to claim 33 wherein said ligand is a steroid, hormone, antiasthmatic, antineoplastic, antiarrhythmic, anticonvulsant, antibiotic, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.

35. A method according to claim 34 wherein R has a molecular weight in a range of 50 to 4000.

36. A method according to claim 35 wherein R has a molecular weight in a range of 100 to 2000.

37. A method according to claim 36 wherein said ligand is an antiarrhythmic.

38. A method according to claim 37 wherein said antiarrhythmic is lidocaine.

39. A method according to claim 38 wherein said ligand is an anticonvulsant.

40. A method according to claim 39 wherein said anticonvulsant is phenytoin.

41. A method according to claim 39 wherein said anticonvulsant is primidone.

42. A method according to claim 39 wherein said anticonvulsant is ethosuximide.

43. A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula:

##STR55## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog is attached to the carboamidosulfonylamino group moiety through a hydroxy oxygen or a reactive amine;

and biologically acceptable salts thereof.

44. A method according to claim 43 wherein the carboamidosulfonylamino group bonded to the R group is also bonded to the 4- or 5-position of the fluorescein moiety.

45. A method according to claim 44 wherein said ligand is a steroid, hormone, antiasthmatic, antineoplastic, antiarrhythmic, anticonvulsant, antibiotic, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.

46. A method according to claim 45 wherein R has a molecular weight in a range of 50 to 4000.

47. A method according to claim 46 wherein R has a molecular weight in a range of 100 to 2000.

48. A method according to claim 47 wherein said ligand is an anticonvulsant.

49. A method according to claim 48 wherein said anticonvulsant is carbamazepine.

50. A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula:

##STR56## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has a reactive nitrogen by which the ligand-analog is attached to the oxalylaminofluorescein moiety;

and biologically acceptable salts thereof.

51. A method according to claim 50 wherein the oxalylamino group bonded to the R group is also bonded to the 4- or 5-position of the fluorescein moiety.

52. A method according to claim 51 wherein said ligand is a steroid, hormone, antiashtmatic, antineoplastic, antiarrhythmic, anticonvulsant, antibiotic, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.

53. A method according to claim 52 wherein R has a molecular weight in a range of 50 to 4000.

54. A method according to claim 53 wherein R has a molecular weight in a range of 100 to 2000.

55. A method according to claim 54 wherein said ligand is an anticonvulsant.

56. A method according to claim 55 wherein said anticonvulsant is carbamazepine.