Ascorbate interference-resistant composition, device and method for the determination of peroxidatively active substances
First Claim
1. In a composition for detecting the presence of a peroxidatively active substance in a test sample, the composition comprising an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of the peroxidatively active substance and peroxide,the improvement wherein the composition additionally comprises a metal chelate of a polycarboxyalkylamine derivative having a general formula:
- ##STR4## where;
(a) R1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms;
R2, R3, Rx and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms;
where at least two of R1, R2, R3, Rx or Ry are alkyl carboxylic acid radicals so defined;
(b) Rp and Rq, same or different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms;
(c) n is an integer having a value of from 0 to 1;
m is an integer having a value of from 0 to 2;
where if m is greater than 0, repeated Rp and repeated Rq radicals may be the same or different; and
(d) M is Fe+3.
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Accused Products
Abstract
A composition, test means (and device) and method for determining peroxidatively active substances in a test sample are disclosed. The composition, test means (and device) and method are rendered resistant to the adverse affects of ascorbate which may be present in the sample by the inclusion in the composition of a metal chelate which is polycarboxyalkylamine derivative having the formula: ##STR1## where: (a) R1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; R2, R3, Rx and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms; where at least two of R1, R2, R3, Rx or Ry are alkyl carboxylic acid radicals so defined;
(b) Rp and Rq, same or different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms;
(c) n is an integer having a value of from 0 to 1; m is an integer having a value of from 0 to 2; where if m is greater than 0, repeated Rp and repeated Rq radicals may be the same or different; and
(d) M is Fe+3.
The composition also comprises an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of peroxide and the peroxidatively active substance. The test means comprises a carrier matrix incorporated with the composition, and the method comprises immersing the test means (or device) in the test sample and observing a color or other detectable response.
66 Citations
23 Claims
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1. In a composition for detecting the presence of a peroxidatively active substance in a test sample, the composition comprising an organic hydroperoxide and an indicator capable of providing a detectable response in the presence of the peroxidatively active substance and peroxide,
the improvement wherein the composition additionally comprises a metal chelate of a polycarboxyalkylamine derivative having a general formula: - ##STR4## where;
(a) R1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms;
R2, R3, Rx and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms;
where at least two of R1, R2, R3, Rx or Ry are alkyl carboxylic acid radicals so defined;(b) Rp and Rq, same or different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (c) n is an integer having a value of from 0 to 1;
m is an integer having a value of from 0 to 2;
where if m is greater than 0, repeated Rp and repeated Rq radicals may be the same or different; and(d) M is Fe+3. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 23)
- ##STR4## where;
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13. A method for preparing a test means for determining the presence of a peroxidatively active substance in a test sample, wherein the test means is resistant to interfering affects of ascorbate which may be present in the sample, which method comprises the steps of:
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(a) preparing a first reagent solution including an organic hydroperoxide, and a metal chelate of an polycarboxyalkylamine derivative having the general formula;
##STR5## where;
(i) R1 is hydrogen or straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms;
R2, R3, Rx and Ry, same or different, are straight or branched chain alkyl alcohol or alkyl carboxylic acid radicals having from 2 to 3 carbon atoms;
where at least two of R1, R2, R3, Rx or Ry are alkyl carboxylic acid radicals so defined;(ii) Rp and Rq, same of different, are straight or branched chain alkylene radicals having from 1 to 3 carbon atoms or divalent 1,2-cycloaliphatic radicals having from 6 to 9 carbon atoms; (iii) n is an integer having a value of from 0 to 1;
m is an integer having a value of from 0 to 2;
where if m is greater than 0, repeated Rp and repeated Rq radicals may be the same or different; and(iv) M is Fe+3 ; (b) incorporating the first reagent solution with a carrier matrix by wetting the matrix with the first solution; (c) drying the wetted matrix to leave a residue of the metal chelate and the hydroperoxide; (d) preparing a second reagent solution including an indicator and a solvent, the indicator being capable of providing a detectable response in the presence of a peroxide and the peroxidatively active substance; (e) incorporating the second reagent solution with the dried carrier matrix by wetting the matrix with the second reagent solution; and (f) drying the matrix to leave a combined residue including the metal chelate, the hydroperoxide and the indicator. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification