Process for saccharification of starch using enzyme produced by fungus belonging to genus Chalara

• US 4,591,560 A
• Filed: 12/29/1983
• Issued: 05/27/1986
• Est. Priority Date: 01/17/1983
• Status: Expired due to Fees

• Current Assignee: Director of National Food Research Institute
• Original Assignee: Director of National Food Research Institute
• Inventors: Kobayashi, Shoichi, Kainuma, Keiji
• Primary Examiner(s): Wiseman, Thomas G.
• Assistant Examiner(s): Huleatt, Jayme A.
• Application Number:
  US06/566,499
• Time in Patent Office: 880 Days
• Field of Search: 435/99, 435/96, 435/201, 435/202, 435/203, 435/911, 435/254, 435/255, 435/205
• US Class Current: 435/96
• CPC Class Codes: C12N 9/2411 Amylases
• CPC Class Codes: C12N 9/242 Fungal source
• CPC Class Codes: C12N 9/2428 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: C12P 19/20 produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
• CPC Class Codes: Y10S 435/911 using fungi

Abstract

A process for the saccharification of starch, which comprises saccharifying a raw and/or gelatinized starch by the use of an amylase produced by a fungus belonging to genus Chalara to produce glucose.

According to the process of the present invention, the starch is directly saccharified, and glucose can be obtained efficiently.

Claims

1. A process for the saccharification of starch to produce glucose, which comprises contacting a starch with an amylase produced by a fungus belonging to the genus Chalara to produce glucose in an aqueous medium having a pH of from 3 to 9.5 at a temperature of from 30°

to 50°

C. to produce said glucose, and recovering said glucose, said amylase has a pH stability of 3-9.5, an optimum temperature of 45°

C. for raw starch and of 50°

C. for gelatinized starch and thermostability of 45°

C.

2. The process of claim 1, wherein said starch is a raw starch.

3. The process of claim 2, wherein said pH is up to 7 and said temperature is between 40°

and 48°

C.

4. The process of claim 3, wherein said pH is between about 3.5 and 6.

5. The process of claim 4, wherein said fungus belonging to the genus Chalara is Chalara paradoxa PNS-80 (FERM BP-422).

6. The process of claim 1, wherein said fungus belonging to the genus Chalara is Chalara paradoxa PNS-80 (FERM BP-422).

7. The process of claim 1, wherein said fungus belonging to the genus Chalara is selected from the group consisting of Chalara quercina and Chalara elegans.

8. The process of claim 1, wherein said aqueous medium also contains calcium ion.

9. A process for producing an amylase which has high activity in digesting starch to form glucose comprising cultivating Chalara paradoxa PNS-80 (FERM BP-422) in a culture medium containing a nitrogen source under aerobic conditions at a pH of 4-8.5 and at a temperature of 25°

-40°

C. to produce said amylase, said amylase has a pH stability of 3-9.5, an optimum temperature of 45°

C. for raw starch and of 50°

C. for gelatinized starch and thermostability of 45°

C.

10. The process of claim 9, wherein said amylase is separated from said culture medium.