Analytical element

• US 4,594,224 A
• Filed: 04/09/1984
• Issued: 06/10/1986
• Est. Priority Date: 01/30/1981
• Status: Expired due to Fees

• Current Assignee: Konishiroku Photo Industry Co., Ltd.
• Original Assignee: Konishiroku Photo Industry Co., Ltd.
• Inventors: Koyama, Mikio, Kikugawa, Shozo, Okaniwa, Kenichiro
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/597,818
• Time in Patent Office: 792 Days
• Field of Search: 422/55-58, 435/805
• US Class Current: 422/428
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

There is presented an improved simple quantitative analytical element for analysis of components in a body fluid such as blood, having a reagent layer constituted of a hydrophilic colloid containing at least one reagent reactive with a component in a liquid sample provided on a support, and also a development layer of a fibrous structure on the reagent layer on the opposite side to the support. A liquid sample can be distributed uniformly through the reagent layer to enable quantitative analysis.

Claims

1. An analytical element, comprising a light-transmissive and liquid-impervious support, at least one reagent layer comprising a hydrophilic colloid containing at least one reagent which reacts with a component in a liquid sample, and at least one development layer having a fibrous structure provided at a position on a side of the reagent layer opposite to that of said support for permitting the component in said liquid sample to permeate into said reagent layer, said development layer having a fibrous structure being formed by coating said reagent layer with a dispersion of fibers wherein said fibers have a size of 50 to 325 mesh.

2. The analytical element of claim 1 wherein the development layer has a thickness of from 30 to 600 microns.

3. The analytical element of claim 1 wherein the support comprises a polymeric material selected from the group consisting of cellulose acetate, polyethylene terephthalate, polycarbonate and polystyrene.

4. The analytical element of claim 3 wherein the support has a thickness of from 50 to 250 microns.

5. The analytical element of claim 1 wherein the hydrophilic colloid is selected from the group consisting of gelatin, gelatin derivatives, polyvinyl alcohol and polyvinyl pyrrolidone.

6. The analytical element of claim 5 wherein the hydrophilic colloid is gelatin.

7. The analytical element of claim 5 wherein the hydrophilic colloid is in the form of a film having a thickness of at least 5 microns.

8. The analytical element of claim 1 wherein the dispersion of fibers is an aqueous dispersion of natural or synthetic fibers.

9. The analytical element of claim 8 wherein said fibers are selected from the group consisting of natural cellulose and derivatives thereof, polyethylene, polypropylene and polyamide.

10. The analytical element of claim 8 wherein the dispersion contains a surfactant.

11. The analytical element of claim 10 wherein the dispersion further contains a polymer binder.

12. The analytical element of claim 10 wherein the amount of said surfactant is from 0.005 to 10% by weight based on the weight of said fibers.

13. The analytical element of claim 10 wherein the surfactant is a nonionic surfactant.

14. The analytical element of claim 13 wherein the dispersion further contains a polymer binder.

15. An analytical element comprising a light-transmissive and liquid-impervious support, at least one reagent layer comprising a hydrophilic colloid selected from the group consisting of gelatin, gelatin derivatives, polyvinyl alcohol and polyvinyl pyrrolidone, having a thickness of at least 5 microns, and at least one reagent which reacts with a component in a liquid sample, and at least one development layer having a fibrous structure, the fibers of said fibrous structure having a size of 50 to 325 mesh and being selected from natural cellulose and derivatives thereof, polyethylene, polypropylene and polyamide, said development layer being provided at a position on a side of the reagent layer opposite to that of said support for permitting the component in said liquid sample to permeate into said reagent layer, said development layer being formed by coating said reagent layer with a dispersion comprising and fibers and a surfactant.