Modified electrode

• US 4,595,479 A
• Filed: 10/19/1983
• Issued: 06/17/1986
• Est. Priority Date: 11/09/1982
• Status: Expired due to Fees

• Current Assignee: Ajinomoto Co., Inc.
• Original Assignee: Ajinomoto Co., Inc.
• Inventors: Kimura, Yasuhiro, Niki, Katsumi
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Chapman, Terryence
• Application Number:
  US06/543,295
• Time in Patent Office: 972 Days
• Field of Search: 204/290 R, 204/294, 204/400
• US Class Current: 204/294
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/008 for determining co-enzymes or co-factors, e.g. NAD, ATP
• CPC Class Codes: G01N 2333/795 Porphyrin- or corrin-ring-containing peptides
• CPC Class Codes: G01N 33/72 involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood

Abstract

A chemically modified electrode comprising a pyrolytic graphite surface, wherein the basal plane of graphite is exposed on said surface and a redox/adsorption electron mediator is irreversibly absorped directly onto the basal plane of graphite on said surface, is disclosed along with methods of using this electrode.

Claims

1. A chemically modified electrode, comprising a pyrolytic graphite surface having a basal plane of graphite which is exposed on the said surface and a non-polymeric redox/adsorption electron mediator irreversibly adsorbed directly onto the said basal plane of graphite on the said surface.

2. The electrode of claim 1, wherein said pyrolytic graphite is stress-annealed pyrolytic graphite.

3. The electrode of claim 1, wherein the said redox/adsorption mediator is at least one member selected from the group consisting of viologen dye, bipyridine, phenanthroline, 1-methoxy-5-methylphenazenium methyl and sulfate N-methylphenazenium methyl sulfate.

4. The electrode of claim 1, wherein said electrode further comprises at least one member selected from the group consisting of NADH, NADPH, cytochrome C₃, cytochrome C, hemoglobin and chlorophyl, conjugated with said redox/adsorption electron mediator.

5. The electrode of claim 1, wherein said electrode further comprises a layer of Anabaena cylindrica on said surface.