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Methods for enumerating 3-part white cell differential clusters

  • US 4,596,035 A
  • Filed: 06/27/1983
  • Issued: 06/17/1986
  • Est. Priority Date: 06/27/1983
  • Status: Expired due to Term
First Claim
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1. A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising:

  • (a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time;

    (b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction;

    (c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement;

    (d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements;

    (e) determining a series of lines orthogonal to said trajectory describable by a nonlinear function; and

    (f) counting the cells along each of said orthogonal lines whereby cellular subpopulations may be enumerated.

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