Monoclonal antibodies which specifically bind to human immune interferon
First Claim
1. As a new composition, a murine-derived hybridoma cell line ATCC HB8291 capable of producing in the medium of its growth an IgG monoclonal antibody capable of specifically binding to human IFN-γ
- in an antigen-antibody complex.
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Accused Products
Abstract
Disclosed are monoclonal antibody substances specifically immunologically reactive with native and recombinant human immune interferon ("IFN-γ") and with polypeptides having amino acid sequences substantially duplicative of sequences extant in IFN-γ. In a presently preferred embodiment, antibody substances are produced by new mouse-mouse hybridoma tumor cell line A.T.C.C. HB 8291 and are immunoreactive with native IFN-γ, with recombinant IFN-γ and polypeptide analogs thereof, and with a nonadecapeptide whose amino acid sequence duplicates that of the final nineteen amino acid residues of the carboxyl terminal of IFN-γ. These preferred antibody substances, while displaying high affinity for IFN-γ, do not neutralize antiviral biological activity of the interferon. They are usefully employed in the detection, quantification and affinity purification of IFN-γ and IFN-γ analogs as well as in investigations relating to the mode of biological action of IFN-γ.
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Citations
4 Claims
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1. As a new composition, a murine-derived hybridoma cell line ATCC HB8291 capable of producing in the medium of its growth an IgG monoclonal antibody capable of specifically binding to human IFN-γ
- in an antigen-antibody complex.
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2. As a new composition, the monoclonal antibody produced from hybridoma cell line ATCC HB8291, said antibody capable of specifically binding to human IFN-γ
- .
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3. In an immunological procedure for isolation of biologically active human IFN-γ
- or polypeptide analogs thereof from a biological fluid comprising the steps of contacting IFN-γ
or polypeptide analogs thereof with an antibody specific for IFN-γ
for a time and under conditions sufficient for formation of an immune complex of said IFN-γ
or polypeptide analogs thereof and said antibody specific for IFN-γ and
isolating said IFN-γ
or polypeptide analogs thereof from said immune complex, the improvement comprising;employing as said specific antibody an IgG monoclonal antibody produced by a murine-derived hybridoma cell line deposited as ATCC No. HB8291, said antibody capable of specifically binding to human IFN-γ
in an antigen-antibody complex.
- or polypeptide analogs thereof from a biological fluid comprising the steps of contacting IFN-γ
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4. In an immunological procedure for the quantitative detection of IFN-γ
- or a polypeptide analog thereof, from a biological fluid comprising the steps of contacting IFN-γ
or polypeptide analogs thereof with an antibody specific for IFN-γ
for a time and under conditions sufficient for formation of an immune complex of said IFN-γ
or polypeptide analogs thereof and said antibody specific for IFN-γ and
detecting the presence of said immune complex, the improvement comprising;employing as said specific antibody of IgG monoclonal antibody produced by a murine-derived hybridoma cell line deposited as ATCC No. HB8291, said antibody capable of specifically binding to human IFN-γ
in an antigen-antibody complex.
- or a polypeptide analog thereof, from a biological fluid comprising the steps of contacting IFN-γ
Specification