Lipoprotein lipase suppression by endotoxin-induced mediator (shock assay)

• US 4,603,106 A
• Filed: 09/07/1982
• Issued: 07/29/1986
• Est. Priority Date: 02/22/1982
• Status: Expired due to Term

• Current Assignee: Rockefeller University
• Original Assignee: Rockefeller University
• Inventors: Cerami, Anthony, Kawakami, Masanobu
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Tarcza, John
• Application Number:
  US06/414,098
• Time in Patent Office: 1,421 Days
• Field of Search: 435/4, 435/7, 435/18, 435/19, 435/177, 435/188, 435/810, 435/184, 435/196, 435/197, 435/198, 435/68, 424/95
• US Class Current: 435/5
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: C07K 14/4703 Inhibitors; Suppressors
• CPC Class Codes: C07K 14/523 Beta-chemokines, e.g. RANTES, I-309/TCA-3, MIP-1alpha, MIP-1beta/ACT-2/LD78/SCIF, MCP-1/MCAF, MCP-2, MCP-3, LDCF-1, LDCF-2
• CPC Class Codes: C07K 16/24 against cytokines, lymphokines or interferons
• CPC Class Codes: C07K 16/241 Tumor Necrosis Factors
• CPC Class Codes: C07K 16/38 against protease inhibitors of peptide structure
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 2501/21 Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
• CPC Class Codes: C12N 2501/22 Colony stimulating factors (G-CSF, GM-CSF)
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12R 2001/91 Cell lines ; Processes using cell lines
• CPC Class Codes: G01N 2333/52 Assays involving cytokines
• CPC Class Codes: G01N 2400/40 Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/975 Kit

Abstract

A mediator substance exhibiting inhibitory effect upon anabolic enzyme activity in mammals, is prepared by a method comprising gathering a sample of macrophage cells from a mammal, incubating a portion of the macrophage cells with a stimulating material that is associated with an invasive event, and inducing the macrophage cells to produce the mediator substance. The stimulator materials, for example, may include endotoxins, trypanosomes and the like, and the enzymes that may be observed range from lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, to the inducers for red blood cells growth and differentiation. The mediator substance may be utilized to raise antibodies which would in turn find utility in testing for the presence or amount of various invasive stimuli, such as infection and the like. Testing procedures and appropriate materials in kit form are also contemplated. The mediator substance may likewise be utilized in procedures as a screening agent, to test for potentially effective anti-shock agents or chemicals.

Claims

1. A proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid synthetase, and capable of inhibiting the growth and differentiation of erythroid-committed cells.

2. A mediator of claim 1 having a molecular weight of about 17,500 Daltons.

3. A mediator of claim 1 labeled with a detectable label.

4. The mediator of claim 3 wherein the label is an enzyme.

5. The mediator of claim 4 wherein the label is selected from the group consisting of peroxidase, B-glucuronidase, B-D-glucosidase, B-D-galactosidase, urease, glucose oxidase plus peroxidase, galactose oxidase plus peroxidase and acid phosphatase.

6. The mediator of claim 3 wherein the label is a chemical which fluoresces when exposed to ultraviolet light.

7. The mediator of claim 6 wherein the chemical is selected from the group consisting of fluorescein, rhodamine, and auramine.

8. The mediator of claim 3 wherein the label is a radioactive element.

9. The mediator of claim 8 wherein the radioactive element is selected from the group consisting of ¹⁴ C, ¹²⁵ I, ¹³¹ I, ³⁵ S and ³ H.

10. A method for preparing a mediator comprising:

A. gathering a sample of macrophage cells from a mammal;

B. incubating a portion of said macrophage cells with a stimulator material associated with an invasive event for a mammal;

C. inducing said macrophage cells to produce said mediator; and

D. recovering a proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid Synthetase, and capable of inhibiting the growth and differtiation of erythroid-committed cells.

11. The method of claim 10 wherein said stimulator material comprises endotoxin.

12. An antibody to a mediator present in the serum of a mammal which has been subjected to invasive stimuli, the mediator to which said antibody is raised comprises a proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the differentiation of erythroid-committed cells.

13. The antibody of claim 12 labeled with a detectable label.

14. The antibody of claim 13 wherein the label is an enzyme.

15. The antibody of claim 14 wherein the label is selected from the group consisting of peroxidase, B-glucuronidase, B-D-glucosidase, B-D-galactosidase, urease, glucose oxidase plus peroxidase, galactose oxidase plus peroxidase, and acid phosphatase.

16. The antibody of claim 13 wherein the label is a chemical which fluoresces when exposed to ultraviolet light.

17. The antibody of claim 16 wherein the chemical is selected from the group consisting of fluorescein, rhodamine, and auramine.

18. The antibody of claim 13 wherein the label is a radioactive element.

19. The antibody of claim 18 wherein the radioactive element is selected from the group consisting of ¹⁴ C, ¹²⁵ I, ¹³¹ I, ³⁵ S and ³ H.

20. The antibody of claim 12 wherein said antibody is present in the serum of a mammal which has been subjected to invasive stimuli.

21. A method for detecting the presence of invasive stimuli in mammals comprising measuring the activity of a proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of supporessing the activity of lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

22. The method of claim 21 wherein said mediator is measured by:

A. preparing at least one sample of said mediator from a corresponding number of distinct known invasive stimuli;

B. preparing a corresponding number of antibodies from said mediator samples;

C. placing a detactable label on a material selected from the group consisting of said mediator samples and said antibodies;

D. placing the labeled material from Step C in contact with a biological sample from said mammal in which invasion is suspected; and

E. examining said biological sample for the presence of said labeled material.

23. The method of claim 21 wherein said mediator is measured by:

A. preparing at least one sample of said mediator from a corresponding number of distinct known invasive stimuli;

B. placing a detectable label on said mediator sample;

C. placing the labeled mediator sample in contact with a biological sample from said mammal in which invasion is suspected; and

D. examining said biological sample for the presence of said labeled mediator sample.

24. A method for measuring the activity of a proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells, said method comprising;

A. preparing the mediator to be measured from a stimulator material known to be associated with said invasive stimulus;

B. forming a labeled material by placing a detectible label on said mediator;

C. isolating a bioligical sample selected from the group consisting of blood and body fluid, from a mammal suspected of harboring said invasive stimulus;

D. placing said labeled material in contact with said biological sample; and

E. examining said biological sample to locate said labeled material, to thereby determine the activity of said mediator.

25. The method of claim 24 further comprising preparing the antibodies which react with said mediator, which antibodies may be used in said method in the same manner as said mediator.

26. The method of claim 25 wherein a second generation of antibodies reactive with the antibodies to said mediator are prepared which may be used in said method in the same manner as said mediator and the antibodies thereto.

27. The method of claim 26 wherein at least one of said antibodies reactive with said mediator is included in said labeled material.

28. The method of claim 27 wherein at least one of said antibodies has placed on it said label.

29. The method of claim:

26 wherein at least one member of said second generation of antibodies is included in said labeled material.

30. The method of claim 29 wherein at least one member of said second generation of antibodies has placed on it said label.

31. The method fo claim 24 wherein said invasive stimulus is an infection.

32. The method df claim 31 wherein said stimulator material comprises an endotoxin.

33. The method of claim 32 wherein said endotoxin comprises lipopolysaccharide.

34. The method of claim 24 wherein said invasive stimulus is selected from the group consisting of bacterial infection, viral infection, protozoan infection, tumorous mammalian cells, and toxins.

35. The method of claim 24 wherein said mediator is prepared by:

A. isolating a colony of mammalian macrophage cells;

B. incubating said macrophage cells with said stimulator material for a period of time sufficient for said stimulator material to induce said macrophage cells to produce said mediator; and

C. isolating said mediator from said macrophage cells.

36. A method of testing the ability of a drug to inhibit the activity of a mediator capable of reducing the lipoprotein lipase activity in the nammalian body which comprises culturing 3T3-L1 cells in a growth media containing the mediator, adding the drug under test and thereafter testing lipoprotein lipase activity of the media, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

37. An assay system for screening drugs and other agents for ability to inhibit production and/or activity of a mediator comprising an observable cellular test colony innoculated with said mediator said mediator being a proteinaceous mediator originating from invasive stimuli treated macrophase cells having the following characteristics;

capable of suppressing the activity of lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

38. A test kit for the demonstration of a mediator in serum or an aqueous medium, comprising:

A. a predetermined amount of at least one labeled immunochemically reactive component obtained by the direct of indirect attachment of a mediator or a specific binding partner thereto, to a detectable label, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells;

B. other reagents; and

C. directions for use of said kit.

39. The test kit of claim 38 wherein the label is an enzyme.

40. A kit useful for detecting the presence in a mammal of a mediator which is capable of reducing lipoprotein lipase activity which comprises at least a mediator substance labeled with a detectable label, wherein said mediator comprises a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

41. A kit useful for detecting the presence in a mammal of a mediator which is capable of reducing lipoprotein lipase activity which comprises at least a mediator antibody labeled with a detectable label, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid snythetase, and capable of inhibiting the growth and differentiation of erythroid-committed cells.

42. A kit useful for detecting the presence in a mammal of a mediator which is capable of reducing lipoprotein lipase activity which comprises at least a mediator antibody and a second antibody which is labeled with a detectable label and is reactive with said mediator antibody, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

capable of suppressing the activity of lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.