Process for cell separation

• US 4,605,558 A
• Filed: 04/20/1984
• Issued: 08/12/1986
• Est. Priority Date: 04/20/1984
• Status: Expired due to Term

• Current Assignee: Wallace Shrimpton
• Original Assignee: Wallace Shrimpton
• Inventors: Shrimpton, Wallace
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US06/602,461
• Time in Patent Office: 844 Days
• Field of Search: 435/2, 424/105, 424/101
• US Class Current: 424/561
• CPC Class Codes: A61K 35/52 Sperm; Prostate; Seminal fluid; Leydig cells of testes
• CPC Class Codes: B03B 5/00 Washing granular, powdered or lumpy materials; Wet separating separating by pneumatic tables or by pneumatic jigs B03B4/00
• CPC Class Codes: B03B 5/30 using heavy liquids or suspensions
• CPC Class Codes: B03D 3/00 Differential sedimentation

Abstract

A process for the separation of X sperm and Y sperm by phenotypic differences in a gravity separation process wherein an osmolality gradient is provided within the separation vessel, the osmolality preferably being in inverse relationship to the density gradient.

Claims

1. In the known process for the separation of X sperm and Y sperm by phenotypic differences in a density separation process wherein sperm are suspended in a nutrient medium having a gradient in density from top to bottom within a vessel, the improvement comprising independently maintaining an osmolality gradient in the nutrient medium within said vessel.

2. The process of claim 1 wherein the osmolality gradient is inverse to that of said density gradient.

3. The process for the preparation of a medium for the gravity separation of cellular biological material enclosed within a semi-permeable membrane, comprising:

a. providing at least one low density first nutrient medium and adjusting the osmolality to a desired value

b. providing at least one high density second nutrient medium and adjusting the osmolality to a lower value than the osmolality of said first nutrient medium,

c. placing said first and second nutrient media in a vessel to provide a gravity separation means having a high density and a low osmolality at one side and a low density and a high osmolality at the other side.

4. A medium for the gravity separation of X and Y spermatozoa wherein said medium has a high density at the bottom and a low density at the top and a low osmolality at the bottom and a high osmolality at the top.