Method of eluting reagent from reagent strips for chemical analyses and reagent strip therefor

• US 4,605,629 A
• Filed: 07/27/1984
• Issued: 08/12/1986
• Est. Priority Date: 12/23/1980
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Werner, Wolfgang, Lange, Hans, Geisler, Edda
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/634,667
• Time in Patent Office: 746 Days
• Field of Search: 422/55-58, 436/166, 436/169, 436/170, 435/805
• US Class Current: 436/166
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

The present invention provides a method of improving reagent elution from a reagent strip comprising a handle on one end which is affixed an absorbent carrier impregnated with a reagent. The absorbent carrier is pressed against the handle by a thin mesh which is stuck or sealed on to the handle on opposite sides of the carrier.

Another mesh is between the carrier and the handle. When the carrier is immersed in a liquid, the meshes improve the elution of the reagent into the liquid.

Claims

1. A method of reagent elution from a reagent strip having an elongated handle and a dry, absorbent carrier impregnated with a reagent on one end of the handle, the method comprising:

providing a thin mesh stuck onto the handle at opposite sides of the absorbent carrier, substantially covering the absorbent carrier, and pressing the absorbent carrier toward the handle and another mesh between the handle and the absorbent carrier for improving the quantitative elution and the rate of elution of the reagent into a liquid when the absorbent carrier is immersed therein; and

immersing the absorbent carrier in the liquid for the elution.

2. The method of claim 1, and further comprising:

making the handle and the mesh of synthetic resin and the absorbent carrier of fleece or paper of at least one material selected from the group consisting of cotton, cellulose, regenerated cellulose, polyamide, and polyester.

3. The method of claim 1, wherein the liquid comprises only an appropriate solvent or diluent.

4. The method of claim 1 for the analysis of a test material, comprising:

after immersing the absorbent carrier in the liquid into which the reagent therein elutes, combining at least part of each of the liquid and test material; and

detecting any physio-chemical change in the combined liquid and test material thereby effected for the analysis of the test material.

5. The method of claim 1 wherein the liquid is a test liquid, and further comprising:

detecting any physio-chemical change in the test liquid thereby effected for the analysis of the test liquid.

6. The method of claim 1, wherein providing the other mesh comprises providing the same with a mesh size in the range of from about 80μ

to about 250μ and

a mesh filament thickness of from about 10μ

to about 50μ

.

7. The method of claim 6, wherein providing the other mesh comprises providing the same with a mesh size in the range of from about 100μ

to about 200μ

.

8. The method of claim 1, and additionally comprising:

providing at least one more such absorbent carrier and meshes on the handle spaced side-by-side with the first-mentioned one.

9. The method of claim 1, and additionally comprising:

providing at least one more absorbent carrier under the thin mesh in sandwich-like fashion with the first-mentioned absorbent carrier, one overlying the other.

10. The method of claim 8 or 9, and further comprising:

providing the absorbent carriers respectively with different reagents.

11. The method of claim 9, and additionally comprising:

providing an intermediate mesh between the overlying absorbent carriers.

12. The method of claim 11, and further comprising:

providing the intermediate mesh with a mesh size in the range of from 80μ

to about 250μ and

a mesh filament thickness of from 10μ

to about 50μ

.

13. The method of claim 12, and further comprising:

providing the other mesh with a mesh size in the range of from about 100μ

to about 200μ

.

14. In a reagent strip having an elongated handle and a dry, absorbent carrier impregnated with a reagent on one end of the handle, the improvement comprising:

means comprising a thin mesh stuck onto the handle at opposite sides of the absorbent carrier, substantially covering the absorbent carrier, and pressing the absorbent carrier toward the handle; and

means comprising another mesh between the handle and the absorbent carrier for improving the quantitative elution and the rate of the elution into the liquid when the absorbent carrier is immersed therein.