Oligonucleotide derivatives

• US 4,605,735 A
• Filed: 02/09/1984
• Issued: 08/12/1986
• Est. Priority Date: 02/14/1983
• Status: Expired due to Term

• Current Assignee: Wakunaga Seiyaku Kabushiki Kaisha
• Original Assignee: Wakunaga Seiyaku Kabushiki Kaisha
• Inventors: Miyoshi, Kenichi, Suzuki, Masanori, Fuwa, Toru
• Primary Examiner(s): Brown, Johnnie R.
• Application Number:
  US06/578,678
• Time in Patent Office: 915 Days
• Field of Search: 536/27, 536/28, 536/29
• US Class Current: 536/26.3
• CPC Class Codes: C07H 19/16 Purine radicals
• CPC Class Codes: C07H 19/20 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

Biotin- or 2,4-dinitrophenyl-oligodeoxyribonucleotide derivatve represented by the formula shown below and its preparation method is disclosed: ##STR1## wherein: R is ##STR2## m and n are each 0 or a natural number; R¹ is a straight or branched hydrocarbon residue; and B is a base constituting the nucleotide (in which when a plural number of B exist, they may be either identical or different). This compound is useful for use as a nonradioactive affinity probe for nucleic acid or a primer.

Claims

1. A nucleotide comprising an oligonucleotide of the formula ##STR12## wherein R is ##STR13## (m+n) is an integer from 1 to 46;

R¹ is a straight or branched C₂ -C₂₀ alkylene; and

each B may be the same or different and is at least one nucleoside residue carrying a base B selected from the group consisting of adenine, cytosine, guanine and thymine.

2. A nucleotide according to claim 1, wherein m is a natural number of 0 to 6, and n is a natural number of 0 to 40.

3. A nucleotide according to claim 1, wherein R is ##STR14##

4. A nucleotide according to claim 1, wherein R is ##STR15##

5. The nucleotide of claim 1 wherein m is 1-4 and n is 0-20.