Self-contained reagent package device for an assay

• US 4,608,231 A
• Filed: 06/03/1985
• Issued: 08/26/1986
• Est. Priority Date: 12/12/1984
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Smith, Roger E., Curry, Robert E., Witty, Thomas R.
• Primary Examiner(s): LACEY, DAVID LYNN
• Application Number:
  US06/740,593
• Time in Patent Office: 449 Days
• Field of Search: 422/57, 422/58, 422/61, 422/101, 422/102, 422/104, 436/165, 436/807, 436/808, 435/300, 435/301, 435/311, 435/810, 206/569, 356/246
• US Class Current: 422/425
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

A self-contained reagent package device for an assay comprises a support member and a plurality of wells in the support member. All of the wells are capable of retaining liquids therein. One of the wells has on its interior surfaces an immunoreactive substance for carrying out the assay. At least one of the wells includes a predetermined amount of reagent therein. Another of the wells is empty so that the specimen to be assayed may be deposited therein. A protective cover is sealed over the open ends of the wells to maintain the incorporated reagents in stable form prior to use. The cover preferably does not seal the open end of one of the wells which serves as a specimen collecting well.

Claims

1. A self-contained reagent package device useful in the performance of chemical and biological assays comprising:

a substantially planar support member;

a plurality of wells in said support member, all of said wells having open top ends for access thereto, and all but one of said wells being integrally formed as a unitary structure with and from the same material as said support member, said one well having its interior surfaces coated with an immunoreactive substance for carrying out the assay;

at least one of said integrally formed wells having a predetermined amount of reagent therein;

another of said integrally formed wells being empty so that the specimen to be assayed may be deposited therein; and

a protective cover sealed over the open ends of said wells to maintain the incorporated reagents in stable form prior to use thereby providing a self-contained reagent package device.

2. The device of claim 1 wherein the open top ends of said wells lie substantially in the same plane.

3. The device of claim 2 wherein said support member is a substantially planar strip having the open top ends of said wells coextensive therewith.

4. The device of claim 3 wherein said wells are linearly arranged in single file in said support member.

5. The device of claim 4 wherein said coated well is positioned at one end of said support member and said empty well for the specimen is positioned at the other end of the support member.

6. The device of claim 1 wherein the protective cover is positioned on the open top ends of the wells such that the empty specimen well is uncovered.

7. The device of claim 1 wherein said support member and all of said wells are made of plastic.

8. The device of claim 7 wherein said coated well is made from a plastic different from the plastic of said integrally formed wells.

9. The device of claim 8 wherein said coated well is made from polystyrene and said integrally formed wells and said support member are made from polypropylene.

10. The device of claim 1 wherein said coated well is made from a material which is substantially optically clear.

11. The device of claim 1 wherein said protective cover includes labeling information thereon relating to the nature of the assay to be performed.

12. The device of claim 11 wherein said information is in the form of a bar code constructed and arranged so as to be read electronically to determine said information.

13. The device of claim 1 wherein the immunoreactive substance on the interior surface of the coated well is antibody.

14. The device of claim 1 wherein the empty well for the specimen has a conically-shaped bottom end.

15. The device of claim 1 which further includes keying means for assuring that the device is insertable into analyzer means in a predetermined direction.

16. The device of claim 1 wherein said coated well has a lip around its open end and said support member has a detent formed therein so that said coated well is snap-fit in said support member.

17. A self-contained reagent package device useful in the performance of chemical and biological assays comprising:

a substantially planar strip member;

a plurality of wells in said strip member, all but one of said wells being integrally formed as a unitary structure with and from the same material as said member;

all of said wells being arranged in single file in said member and having open top ends for access thereto with said open top ends lying in substantially the same plane coextensive with the member, the non-integrally formed well being positioned at one end of said member and made from substantially optically clear material and having its interior surfaces coated with an immunoreactive substance for carrying out the assay;

at least one of said integrally formed wells having a predetermined amount of reagent therein;

one of said integrally formed wells at the other end of the member being empty so that the specimen to be assayed may be deposited therein, said specimen well having a conically-shaped bottom end; and

a protective cover sealed over the open ends of said wells except said empty specimen well to maintain the incorporated reagents in stable form prior to use, said cover including labeling information thereon in the form of a bar code constructed and arranged so as to be read electronically to determine said information.