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Method of growing a confluent layer of cells on a porous or semi-permeable substrate and apparatus for practicing the method

  • US 4,608,342 A
  • Filed: 05/14/1984
  • Issued: 08/26/1986
  • Est. Priority Date: 05/13/1983
  • Status: Expired due to Fees
First Claim
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1. A method for growing a confluent layer of cells on a substrate surface, comprising the steps of:

  • providing a porous or semi-permeable substrate in the form of a tensioned, taut, planar substrate having exposed upper and lower surfaces and independently held in such configuration by a tensioning and holding device;

    providing a receptacle containing fluid nutrient medium for cell growth, said receptacle having a bottom surface and upstanding side walls about the periphery thereof;

    arranging within said receptacle a body of solid, plate material having a bottom surface in contact with the bottom surface of said receptacle and an upper solid, planar surface extending above the plane of the bottom surface of said receptacle;

    arranging said holding and tensioning device within said receptacle such that the lower surface of said substrate is in contact with the upper non-porous surface of said plate material and such that the substrate is surrounded by the fluid nutrient medium for cell growth;

    providing on the upper surface of said substrate a source of cells; and

    maintaining said arrangement under conditions, and for a time, effective to produce a confluent layer of cells on the upper surface of said substrate, whereby a region is created created between the mutually contacting surfaces of said substrate and said plate material and in said region substances synthesized by the cells in aid of their anchoring to said substrate and passing through the porous or semi-permeable substrate are enriched proximate to the cells and not substantially decomposed, modified or diluted by the surrounding nutrient medium.

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