Optical continuous measuring apparatus for reaction-liquid

• US 4,622,208 A
• Filed: 05/03/1985
• Issued: 11/11/1986
• Est. Priority Date: 05/11/1984
• Status: Expired due to Fees

• Current Assignee: Eisai Company Limited (Eisai Medical Research Incorporated)
• Original Assignee: Eisai Company Limited (Eisai Medical Research Incorporated)
• Inventors: Namba, Yuzaburo, Takahashi, Toshio
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/730,035
• Time in Patent Office: 557 Days
• Field of Search: 422/63, 422/64, 422/65, 422/67, 422/100, 422/102, 73/864.81, 141/67, 356/246
• US Class Current: 422/65
• CPC Class Codes: G01N 35/02 using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations G01N35/0098 and G01N35/0099 take precedence
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An optical continuous measuring apparatus for a reaction-liquid comprises: a microplate provided with a plurality of microcups arranged on a straight line at intervals of a certain distance; a rotary disc provided over the microplate so as to be rotatable, which disc is provided with suction nozzles in its outer periphery, which nozzles are sequentially inserted into the microcups upon rotation of the disc, and also provided with transparent measuring cells communicating therewith inward in radial direction of the disc; suction means for sucking the reaction-liquid received in the microcups from the microcups into the measuring cells through the suction nozzles when the suction nozzles of the rotary disc are inserted into the microcups; an optical measuring means for measuring the reaction-liquid received in the measuring cells; a supplying means for supplying a rinsing liquid to the measuring cells; and a discharging means for discharging the reaction-liquid and the rinsing liquid from the measuring cells.

Claims

1. An optical continuous measuring apparatus for reaction liquid comprising:

a plate-like member provided with a plurality of cavities for receiving a reaction-liquid, said plate-like member being movable in a first direction and a second direction, opposite to said first direction, said cavities being disposed linearly, parallel to said first direction and spaced apart from one another in said first direction by a predetermined uniform distance;

a first dirving means for intermittently moving said plate-like member by said predetermined uniform distance in said first direction;

a disk-shaped rotary member, having front and back side surfaces, a periphery and an axis of rotation transverse to said first direction, provided over said platelike member so as to be rotatable in said first direction;

a plurality of transparent measuring cells receivable of said reaction liquid, each cell having an inlet and an outlet, radially disposed on said rotary member, said inlets being disposed radially outwards of said outlets;

a plurality of suction nozzles projecting radially outward from said rotary member, each suction nozzle corresponding to a respective measuring cell and having a first end and a second end, said second ends being disposed radially inwards of said first ends and being in fluid communication with said inlet of said respective measuring cell, said first ends being circumferentially spaced apart from each other by a distance substantially equal to or slightly greater than said predetermined uniform distance, said first ends being receivable within respective cavities of said plurality of cavities in said plate-like member;

a second driving means for intermittently rotating said rotary member in said first direction such that each of said first ends of said plurality of suction nozzles move a circumferential distance substantially equal to said predetermined uniform distance, said second driving means cooperating with said first driving means so that upon intermittent rotation of said rotary member in said first direction each of said suction nozzles is in turn received within a respective one of said cavities as said plate-like member is intermittently moved in said first direction;

suction means, selectively connectable to said measuring cells, for applying a negative pressure to the outlet of the measuring cell corresponding to the suction nozzle whose first end is received within said respective cavity so as to draw reaction liquid into said measuring cell;

measuring means for optically measuring the reaction liquid received in said measuring cell;

a rinsing liquid supplying/discharging means, connectable to said measuring cells, for supplying a rinsing liquid to said measuring cells and discharging said rinsing liquid from said measuring cells.

2. The optical continuous measuring apparatus for reaction liquid as set forth in claim 1, wherein:

said outlets of said measuring cells individually open to a side surface of said rotary member;

said suction means comprises a suction passage provided in a supporting plate which has a side surface thereof hermetically brought into slidable contact with said backside surface of said rotary member, said suction passage opening, through said side surface of said supporting plate, in a position communicating, in turn, with each of said outlets of said measuring cells upon rotation of said rotary member.

3. The optical continuous measuring apparatus for reaction liquid as set forth in claim 1, wherein:

said rinsing liquid supplying/discharging means comprises a rinsing liquid supply pipe communicable, in turn, with each of said first ends of said suction nozzles upon rotation of said rotary member; and

a liquid discharging pipe communicable, in turn, with each of said outlets of said measuring cells upon rotation of said rotary member.

4. The optical continuous measuring apparatus for reaction liquid as set forth in claim 3, wherein

said rinsing liquid supply pipe has a front end and a slit formed in said front end, said slit having a width sufficient to enable said first ends of said suction nozzles to pass through said slit, in turn, upon rotation of said rotary member.

5. The optical continuous measuring apparatus for reaction liquid as set forth in claim 3, wherein:

said liquid discharging pipe comprises means defining an opening communicable, in turn, with each of said outlets of said measuring cells upon rotation of said rotary member, said opening means being arc-shaped and formed in a side surface of a supporting member.

6. The optical continuous measuring apparatus for reaction liquid as set forth in claim 1, wherein

said plate-like member is further movable in a third direction, which is transverse to said first direction, and a fourth direction, which is opposite to said third direction;

and

said suction nozzles are provided only on a portion of the outer periphery of said rotary member whereby said plate-like member in its movement in said second, third and fourth directions cooperates with a remaining portion of the outer periphery of said rotary member so that said suction nozzles do not abut against said plate-like member and the plate-like member can be moved free of said suction nozzles.

7. The optical continuous measuring apparatus for reaction liquid as set forth in claim 1, wherein

said second ends of said suction nozzles are each received within the inlet of a respective measuring cell, said second ends each being in the form of a hollow closed cylinder having an axis, said second end being provided with a plurality of spaced apart radial through holes in fluid communication with said measuring cell.