Sample collector and assay device and method for its use

• US 4,624,929 A
• Filed: 12/03/1984
• Issued: 11/25/1986
• Est. Priority Date: 12/03/1984
• Status: Expired due to Fees

• Current Assignee: Syntec Incorporated (Atos SE)
• Original Assignee: Syntec Incorporated (Atos SE)
• Inventors: Ullman, Edwin F.
• Primary Examiner(s): Lacey, David L.
• Application Number:
  US06/677,144
• Time in Patent Office: 722 Days
• Field of Search: 422/56, 422/58, 422/59, 422/61, 422/70, 422/100, 422/60, 73/864.17, 73/864.54, 73/864.72, 73/864.91, 436/162, 436/165, 436/174, 436/179
• US Class Current: 436/179
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: Y10S 435/962 Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
• CPC Class Codes: Y10S 435/966 involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
• CPC Class Codes: Y10T 436/25625 Dilution

Abstract

A device is disclosed for collecting a liquid sample and diluting the liquid sample by virtue of being adapted for use with a container having a volume of diluting liquid. The device comprises a housing adapted for mating with the container where the mated housing and container form a chamber. A bibulous pad is attached to the housing for collecting a predetermined amount of a liquid sample. Additionally, the device, when used with the container, creates a pressure differential between the interior of the housing and the chamber. The pressure differential is sufficient to move a predetermined volume of the diluting liquid through the bibulous pad and into the housing. The device can be used for assaying for a component of a sample. The device has particular use in immunochromatography and for convenience can also contain a strip of bibulous material confined in the housing. In a method for collecting a liquid sample and subsequently diluting the sample with a predetermined volume of a liquid, the sample is collected on an bibulous pad at the base of a housing. The pad is designed to absorb a predetermined amount of the sample. Next, a pressure differential is created between the housing and a container having a volume of diluting liquid to cause a predetermined volume of the liquid to pass through the pad and into the housing.

Claims

1. A device for collecting a liquid sample, which device comprises:

a housing,

means attached to said housing for collecting a predetermined amount of a liquid sample, said means comprising a bibulous pad designed to absorb and hold said predetermined amount of liquid sample, and

means attached to said housing for mating said device with a container having a volume of liquid for dilution of said liquid sample and during said mating creating a pressure differential between the interior of said housing and a chamber formed by said mated housing and container, said pressure differential being sufficient to move a predetermined volume of the liquid through said means attached to said housing for collecting a predetermined amount of a liquid sample and into said housing.

2. The device of claim 1 in which further includes a strip of bibulous material confined in said housing.

3. The device of claim 2 wherein said strip of bibulous material is a paper strip.

4. The device of claim 2 which further includes means within said housing for maintaining said strip of bubulous material free from contact with said housing.

5. The device of claim 2 wherein said strip of bibulous material contains reagents for performing a chromatographic test.

6. The device of claim 2 wherein said strip of bibulous material contains reagents for performing an immunochromatography.

7. A kit comprising in combination:

(a) the device of claim 2,

(b) a container capable of mating with the device of claim 2, and

(c) reagents for conducting a chromatographic test.

8. The kit of claim 7 wherein the reagents are immunochromatographic test conducting reagents.

9. The device of claim 1 which further includes means for removing excess liquid and filtering out particles from said sample, said means being removable, porous and in overlying relationship to said sample collecting means.

10. The device of claim 1 wherein said housing comprises a continuous side wall and top and bottom walls.

11. The device of claim 1 wherein said means for mating includes an air-tight seal.

12. The device of claim 11 wherein said seal is an O-ring.

13. The device of claim 1 wherein said housing includes means for venting the interior of said housing to the outside atmosphere.

14. The device of claim 1 which further includes an opening in the bottom of said housing.

15. The device of claim 1 wherein said means for mating includes means for concomitantly sealing said housing in the container and forcing a volume of air in said chamber into said housing, said volume of air being sufficient to force the

16. In combination, a container having a predetermined volume of liquid for diluting a liquid sample and a device for collecting a liquid sample, which device comprises:

a housing,

means attached to said housing for sealed mating of said device with said container,

means incorporated with said housing for providing an air-tight seal during the mating of said housing and said container,

means attached to said housing for collecting a predetermined amount of a sample, said means comprising a bibulous pad designed to absorb and hold said predetermined amount of liquid sample,

means for venting the interior of said housing to the outside atmosphere, and

means incorporated into said housing for allowing the liquid to enter said housing,

the dimensions of said housing in conjunction with the dimensions of said container being such that upon sealed mating of said housing and said container a volume of air between the inner walls of said container and the outer walls of said housing forces substantially all of the predetermined volume of the liquid through said means attached to said housing for collecting a predetermined amount of a sample and into said housing through said means incorporated into said housing for allowing the liquid to enter said housing.

17. The device of claim 16 which further includes a strip of bibulous material confined in said housing.

18. The device of claim 17 which further includes means within said housing for maintaining the strip of bibulous material free from contact with said housing.

19. The device of claim 16 which further includes means for removing excess liquid and filtering out cells and cellular debris, said means being removable, porous, and in overlying relationship to said sample collecting means.

20. In combination a device for collecting a liquid sample and a vial containing a volume of a liquid medium comprising reagents for a chromatographic test, said vial having threaded closure means, said device comprising:

a housing formed by a continuous side wall and top and bottom walls,

a bibulous pad attached to the outside of the bottom wall of said housing, said bibulous pad being designed to absorb and hold a predetermined amount of a liquid sample,

an opening to the outside atmosphere in the top wall of said housing, and

an opening in the bottom wall of said housing permitting fluid communication through said bibulous pad to the interior of said housing,

said top wall having threaded means compatible with the threaded closure means of said vial, said threaded means including means for forming an air-tight seal between said housing and said vial,

the dimensions of said housing and said vial being such that upon the sealed closure of said housing into said vial said bibulous pad intimately contacts the bottom of said vial and a volume of air is trapped between said housing and said container sufficient to force a predetermined volume of liquid medium in said vial for diluting said liquid sample through said bibulous pad and concomitantly into said housing.

21. The device of claim 20 which further includes a paper strip confined in said housing.

22. The device of claim 21 which further includes a circumferential protrusion in an interior bottom portion of said housing, said protrusion creating a void in the bottom portion of said housing.

23. The device of claim 20 which further includes a removable, porous pad in overlying relationship to said bibulous pad.

24. A device for diluting a liquid sample, which comprises:

a first chamber formed by side and top and bottom walls,

a bibulous pad attached to the outside of the bottom wall of said first chamber, said bibulous pad being designed to absorb and hold a predetermined amount of a liquid sample,

an opening to the outside atmosphere in the top wall of said first chamber, and

an opening in the bottom wall of said first chamber permitting fluid communication through said pad to the interior of said first chamber,

a second chamber containing a volume of a liquid, said second chamber formed by side and bottom walls,

means incorporated in said top wall of said first chamber for sealing said second chamber so as to force a volume of air into said first chamber, said volume of air being sufficient to move a predetermined volume of liquid through said pad and into said first chamber thereby diluting said sample.

25. The device of claim 24 which further comprises a paper strip confined in said first chamber.

26. The device of claim 25 which further includes a circumferential protrusion in an interior bottom portion of said first chamber to maintain said strip free from contact with the inner bottom wall of said first chamber.

27. The device of claim 24 which further includes a removable porous pad in overlying relationship to said bibulous pad.

28. A method for diluting a liquid sample with a predetermined volume of a liquid, which comprises:

(a) collecting said sample on a bibulous pad at the base of a housing, said pad being designed to absorb and hold a predetermined amount of said sample, and

(b) creating a pressure differential between said housing and a container having a volume of liquid for dilution of said liquid sample to cause a predetermined volume of said liquid to pass through said pad and move into the housing, said sample or a component thereof being soluble in said liquid.

29. The method of claim 28 wherein said pressure differential is created by concomitantly sealing the container and trapping a volume of air sufficient to force said predetermined volume of liquid through said pad.

30. The method of claim 28 wherein the housing contains a strip of bibulous material and the predetermined volume of liquid, with the sample or a component thereof dissolved therein, contacts a portion of the strip.

31. The method of claim 30 wherein the strip of bibulous material is maintained free from contact with the inner bottom wall of said housing.

32. The method of claim 30 wherein said strip of bibulous material is a paper strip.

33. The method of claim 30 wherein said strip of bibulous material contains reagents for performing a chromatographic test.

34. The method of claim 30 wherein said strip of bibulous material contains reagents for performing an immunochromatography.

35. The method of claim 28 wherein said liquid sample contains particles and said particles are removed therefrom prior to creating the pressure differential.

36. The method of claim 28 wherein the housing contains a continuous side wall and top and bottom walls.

37. The method of claim 28 wherein an air-tight seal is employed to create said pressure differential.

38. In a kit containing reagents for conducting an immunochromatography, the improvement which comprises included with said kit a device for collecting a liquid sample, which device comprises:

a housing

a strip of bibulous material confined in said housing,

means attached to said housing for collecting a predetermined amount of a liquid sample, said means comprising a bibulous pad designed to absorb and hold said predetermined amount of liquid sample, and

means attached to said housing for mating said device with a container having a volume of liquid for dilution of the liquid sample and during said mating creating a pressure differential between the interior of said housing and a chamber formed by said mated housing and container, said pressure differential being sufficient to move a predetermined volume of the liquid through said means attached to said housing for collecting a predetermined amount of a liquid sample and into said housing.