Labeled DNA

• US 4,626,501 A
• Filed: 09/02/1983
• Issued: 12/02/1986
• Est. Priority Date: 09/02/1983
• Status: Expired due to Fees

• Current Assignee: Genzyme Corporation (Sanofi )
• Original Assignee: Integrated Genetics, Inc. (Laboratory Corporation Of America Holdings)
• Inventors: Landes, Greg M.
• Primary Examiner(s): Nucker, Christine M.
• Application Number:
  US06/529,044
• Time in Patent Office: 1,187 Days
• Field of Search: 536/23, 536/26, 424/251, 424/253, 544/264, 544/269, 544/272, 544/280, 544/281, 435/6, 436/504, 436/508, 436/518, 436/528, 436/800, 436/804, 436/823, 935/77, 935/78
• US Class Current: 435/6.12
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2525/117 incorporating modified base
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2565/1025 labels being on the same oligonucleotide
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Abstract

Polymeric, single-stranded DNA molecule which is labeled with a plurality of labels, the labels being bonded, via linking groups, to adenine and cytosine bases of the DNA molecule in substantially equal numbers, the labels being bonded to essentially none of the guanine or thymine bases of the DNA molecule, the DNA molecule being adapted for use as a probe in a nucleic acid hybridization assay.

Claims

1. A polymeric DNA molecule wherein at least some of the adenine and cytosine bases of said DNA molecule are derivatized to their respective etheno analogs, said analogs having the formula ##STR11## wherein n is between 0 and 10, inclusive;

each p is between 1 and 10, inclusive;

n_a is N-1 of adenine or N-3 of cytosine;

N_b is N-6 of adenine or N-4 of cytosine;

B is the remainder of the adenine or cytosine base;

W is SO₂ --(CH₂)_q, q being between 1 and 10, inclusive; and

G is X, X being NH--(CH₂)_r --Z wherein 1≦

r≦

10, Z being a detectable entity.

2. The DNA molecule of claim 1 wherein n is 0, p is 2, and W is SO₂ --(CH₂)_q, wherein q is 2.

3. The DNA molecule of claim 1 wherein at least half of the adenine bases and at least half of the cytosine bases of said DNA molecule are derivatized.

4. The DNA molecule of claim 1 wherein G is X, X comprising one of a specific binding pair.

5. The DNA molecule of claim 1 wherein G is X, X comprising a detectable enzyme.

6. The DNA molecule of claim 1 wherein G is X, X comprising a detectable hapten.

7. The DNA molecule of claim 1 wherein G is X, X comprising a radioactively detectable atom.

8. The DNA molecule of claim 1 wherein G is X, X comprising a spectro photometrically detectable molecule.

9. The DNA molecule of claim 1 wherein G is X, X comprising a fluorescent molecule.

10. The DNA molecule of claim 1 wherein Z is biotin or an avidin-specific binding derivative thereof.

11. The DNA molecule of claim 10 wherein said specific binding derivative of biotin is ##STR12##

12. The DNA molecule of claim 4 wherein said one of a specific binding pair is one of a specific binding immunological pair.

13. The DNA molecule of claim 12 wherein said one of a specific binding immunological pair is one of an antibody/antigen pair.

14. The DNA molecule of claim 1 wherein Z is a fluorescent molecule.

15. The DNA molecule of claim 1 wherein Z is a spectro photometrically detectable molecule.

16. The DNA molecule of claim 1 wherein Z is a radioactively detectable atom.

17. The DNA molecule of claim 1 wherein said etheno analogs of said DNA molecule have the formula:

##STR13##

18. The DNA molecule of claim 1 wherein said etheno analogs of said DNA molecule have the formula:

##STR14##

19. The DNA molecule of claim 1 wherein said etheno analogs of said DNA molecule have the formula:

##STR15## wherein r is between 1 and 10, inclusive.

20. The DNA molecule of claim 1 wherein said etheno analogs of said DNA molecule have the formula:

##STR16## wherein r is between 1 and 10, inclusive.

21. A polymeric DNA molecule wherein at least some of the adenine and cytosine basis of said DNA molecule are derivatized to their respective etheno analogs having the formula:

##STR17## wherein N_a is N-1 of adenine or N-3 of cytosine;

N_b is N-6 of adenine or N-4 of cytosine; and

B is the remainder of said adenine or cytosine base.

22. A method of making a probe for use in a nucleic acid hybridization assay for a specific nucleic acid sequence, said method comprising

providing a single-stranded polymeric DNA molecule capable of specifically hybridizing with said nucleic acid sequence,

reacting said DNA molecule with a compound of the formula ##STR18## wherein V is a halogen;

n is between 0 and 10, inclusive; and

each p and q, independently, is between 1 and 10, inclusive, whereby at least some of the adenine and cytosine bases of said DNA molecule are derivatized to their respective etheno analogs, said analogs having the formula ##STR19## wherein n is between 0 and 10, inclusive;

each p and q, independently, is between 1 and 10, inclusive;

N_a is N-1 of adenine or N-3 of cytosine;

N_b is N-6 of adenine or N-4 of cytosine; and

B is the remainder of the adenine or cytosine base,

performing one or more steps to substitute, for ##STR20## on said analogs, a substituent comprising a label which either is capable of being detected or is capable of selectively bonding to an indicator to form a detectable complex.

23. A polymeric, single-stranded DNA molecule adapted for use as a probe in a nucleic acid hybridization assay for a specific nucleic acid sequence, said molecule comprising

a first portion capable of specifically hybridizing with said specific nucleic acid sequence, and

a second tail portion not capable of so hybridizing,

said tail portion being labeled with a plurality of labels and said first portion being substantially unlabeled wherein said tail contains adenine or cytosine bases, and is substantially free of guanine and thymine bases, and

wherein at least some of said adenine or cytosine bases have bonded to them a substituent of the formula ##STR21## wherein n is between 0 and 10, inclusive;

each p and q, independently, is between 1 and 10, inclusive;

N_a is N-1 of adenine or N-3 of cytosine;

N_b is N-6 of adenine or N-4 of cytosine;

B is the remainder of the adenine or cytosine base; and

X is NH--(CH₂)_r --Z, wherein 1≦

r≦

10 and Z is a detectable chemical entity.

24. A method of making a probe for use in a nucleic acid hybridization assay for a specific nucleic acid sequence, said method comprising

providing a single-stranded polymeric DNA molecule capable of specifically hybridizing with said nucleic acid sequence,

reacting said DNA molecule with a compound of the formula ##STR22## wherein V is a halogen;

n is between 0 and 10, inclusive; and

each p and q, independently, is between 1 and 10, inclusive, whereby at least some of the adenine and cytosine bases of said DNA molecule are derivatized to etheno analogs,

performing one or more steps to substitute, for ##STR23## on said analogs, a substituent comprising a label which either is capable of being detected or is capable of selectively bonding to an indicator to form a detectable complex.